1zs4: Difference between revisions
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[[Image:1zs4.gif|left|200px]] | [[Image:1zs4.gif|left|200px]] | ||
'''Structure of bacteriophage lambda cII protein in complex with DNA''' | {{Structure | ||
|PDB= 1zs4 |SIZE=350|CAPTION= <scene name='initialview01'>1zs4</scene>, resolution 1.7Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= CII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Enterobacteria phage lambda]) | |||
}} | |||
'''Structure of bacteriophage lambda cII protein in complex with DNA''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1ZS4 is a [ | 1ZS4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZS4 OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of bacteriophage lambda cII and its DNA complex., Jain D, Kim Y, Maxwell KL, Beasley S, Zhang R, Gussin GN, Edwards AM, Darst SA, Mol Cell. 2005 Jul 22;19(2):259-69. PMID:[http:// | Crystal structure of bacteriophage lambda cII and its DNA complex., Jain D, Kim Y, Maxwell KL, Beasley S, Zhang R, Gussin GN, Edwards AM, Darst SA, Mol Cell. 2005 Jul 22;19(2):259-69. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16039594 16039594] | ||
[[Category: Enterobacteria phage lambda]] | [[Category: Enterobacteria phage lambda]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: transcription activator]] | [[Category: transcription activator]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:39:12 2008'' | ||
Revision as of 16:39, 20 March 2008
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| , resolution 1.7Å | |||||||
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| Gene: | CII (Enterobacteria phage lambda) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of bacteriophage lambda cII protein in complex with DNA
OverviewOverview
The tetrameric cII protein from bacteriophage lambda activates transcription from the phage promoters P(RE), P(I), and P(AQ) by binding to two direct repeats that flank the promoter -35 element. Here, we present the X-ray crystal structure of cII alone (2.8 A resolution) and in complex with its DNA operator from P(RE) (1.7 A resolution). The structures provide a basis for modeling of the activation complex with the RNA polymerase holoenzyme, and point to the key role for the RNA polymerase alpha subunit C-terminal domain (alphaCTD) in cII-dependent activation, which forms a bridge of protein/protein interactions between cII and the RNA polymerase sigma subunit. The model makes specific predictions for protein/protein interactions between cII and alphaCTD, and between alphaCTD and sigma, which are supported by previous genetic studies.
About this StructureAbout this Structure
1ZS4 is a Single protein structure of sequence from Enterobacteria phage lambda. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of bacteriophage lambda cII and its DNA complex., Jain D, Kim Y, Maxwell KL, Beasley S, Zhang R, Gussin GN, Edwards AM, Darst SA, Mol Cell. 2005 Jul 22;19(2):259-69. PMID:16039594
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