1z0u: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1z0u.gif|left|200px]] | [[Image:1z0u.gif|left|200px]] | ||
'''Crystal structure of a NAD kinase from Archaeoglobus fulgidus bound by NADP''' | {{Structure | ||
|PDB= 1z0u |SIZE=350|CAPTION= <scene name='initialview01'>1z0u</scene>, resolution 2.00Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=NAP:NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE'>NAP</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/NAD(+)_kinase NAD(+) kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.23 2.7.1.23] | |||
|GENE= ppnK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2234 Archaeoglobus fulgidus]) | |||
}} | |||
'''Crystal structure of a NAD kinase from Archaeoglobus fulgidus bound by NADP''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1Z0U is a [ | 1Z0U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z0U OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structures of an NAD kinase from Archaeoglobus fulgidus in complex with ATP, NAD, or NADP., Liu J, Lou Y, Yokota H, Adams PD, Kim R, Kim SH, J Mol Biol. 2005 Nov 25;354(2):289-303. Epub 2005 Sep 30. PMID:[http:// | Crystal structures of an NAD kinase from Archaeoglobus fulgidus in complex with ATP, NAD, or NADP., Liu J, Lou Y, Yokota H, Adams PD, Kim R, Kim SH, J Mol Biol. 2005 Nov 25;354(2):289-303. Epub 2005 Sep 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16242716 16242716] | ||
[[Category: Archaeoglobus fulgidus]] | [[Category: Archaeoglobus fulgidus]] | ||
[[Category: NAD(+) kinase]] | [[Category: NAD(+) kinase]] | ||
| Line 31: | Line 40: | ||
[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
[[Category: psi]] | [[Category: psi]] | ||
[[Category: structural | [[Category: structural genomic]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:29:54 2008'' | ||
Revision as of 16:29, 20 March 2008
| |||||||
| , resolution 2.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | ppnK (Archaeoglobus fulgidus) | ||||||
| Activity: | NAD(+) kinase, with EC number 2.7.1.23 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a NAD kinase from Archaeoglobus fulgidus bound by NADP
OverviewOverview
NAD kinase is a ubiquitous enzyme that catalyzes the phosphorylation of NAD to NADP using ATP or inorganic polyphosphate (poly(P)) as phosphate donor, and is regarded as the only enzyme responsible for the synthesis of NADP. We present here the crystal structures of an NAD kinase from the archaeal organism Archaeoglobus fulgidus in complex with its phosphate donor ATP at 1.7 A resolution, with its substrate NAD at 3.05 A resolution, and with the product NADP in two different crystal forms at 2.45 A and 2.0 A resolution, respectively. In the ATP bound structure, the AMP portion of the ATP molecule is found to use the same binding site as the nicotinamide ribose portion of NAD/NADP in the NAD/NADP bound structures. A magnesium ion is found to be coordinated to the phosphate tail of ATP as well as to a pyrophosphate group. The conserved GGDG loop forms hydrogen bonds with the pyrophosphate group in the ATP-bound structure and the 2' phosphate group of the NADP in the NADP-bound structures. A possible phosphate transfer mechanism is proposed on the basis of the structures presented.
About this StructureAbout this Structure
1Z0U is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structures of an NAD kinase from Archaeoglobus fulgidus in complex with ATP, NAD, or NADP., Liu J, Lou Y, Yokota H, Adams PD, Kim R, Kim SH, J Mol Biol. 2005 Nov 25;354(2):289-303. Epub 2005 Sep 30. PMID:16242716
Page seeded by OCA on Thu Mar 20 15:29:54 2008