3dw4: Difference between revisions

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-[[Image:3dw4.png|left|200px]]
+==Crystal Structure of the Sarcin/Ricin Domain from E. COLI 23 S rRNA, U2650-OCH3 modified==
+<StructureSection load='3dw4' size='340' side='right' caption='[[3dw4]], [[Resolution|resolution]] 0.97&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3dw4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DW4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DW4 FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1q9a|1q9a]], [[3dvz|3dvz]], [[3dw5|3dw5]], [[3dw6|3dw6]], [[3dw7|3dw7]], [[483d|483d]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dw4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dw4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3dw4 RCSB], [http://www.ebi.ac.uk/pdbsum/3dw4 PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Site-specific 2'-methylseleno RNA labeling is a promising tool for tackling the phase problem in RNA crystallography. We have developed an efficient strategy for crystallization and structure determination of RNA and RNA/protein complexes based on preliminary crystallization screening of 2'-OCH(3)-modified RNA sequences, prior to the replacement of 2'-OCH(3) groups with their 2'-SeCH(3) counterparts. The method exploits the similar crystallization properties of 2'-OCH(3)- and 2'-SeCH(3)-modified RNAs and has been successfully validated for two test cases. In addition, our data show that 2'-SeCH(3)-modified RNA have an increased resistance to X-ray radiolysis in comparison with commonly used 5-halogen-modified RNA, which permits collection of experimental electron density maps of remarkable quality.
-{{STRUCTURE_3dw4|  PDB=3dw4  |  SCENE=  }}
+A fast selenium derivatization strategy for crystallization and phasing of RNA structures.,Olieric V, Rieder U, Lang K, Serganov A, Schulze-Briese C, Micura R, Dumas P, Ennifar E RNA. 2009 Apr;15(4):707-15. Epub 2009 Feb 18. PMID:19228585<ref>PMID:19228585</ref>
-===Crystal Structure of the Sarcin/Ricin Domain from E. COLI 23 S rRNA, U2650-OCH3 modified===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_19228585}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[3dw4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DW4 OCA].
+</StructureSection>
+[[Category: Dumas, P]]
-==Reference==
+[[Category: Ennifar, E]]
-<ref group="xtra">PMID:019228585</ref><references group="xtra"/>
+[[Category: Lang, K]]
-[[Category: Dumas, P.]]
+[[Category: Micura, R]]
-[[Category: Ennifar, E.]]
+[[Category: Olieric, V]]
-[[Category: Lang, K.]]
+[[Category: Rieder, U]]
-[[Category: Micura, R.]]
+[[Category: Schulze-Briese, C]]
-[[Category: Olieric, V.]]
+[[Category: Serganov, A]]
-[[Category: Rieder, U.]]
-[[Category: Schulze-Briese, C.]]
-[[Category: Serganov, A.]]
 [[Category: Rna]]
 [[Category: Sarcin ricin loop]]