1xnj: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1xnj.gif|left|200px]] | [[Image:1xnj.gif|left|200px]] | ||
'''APS complex of human PAPS synthetase 1''' | {{Structure | ||
|PDB= 1xnj |SIZE=350|CAPTION= <scene name='initialview01'>1xnj</scene>, resolution 1.98Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=ADX:ADENOSINE-5'-PHOSPHOSULFATE'>ADX</scene>, <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene> and <scene name='pdbligand=ADX:ADENOSINE-5'-PHOSPHOSULFATE'>ADX</scene> | |||
|ACTIVITY= | |||
|GENE= PAPSS1, PAPSS, ATPSK1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |||
}} | |||
'''APS complex of human PAPS synthetase 1''' | |||
==Overview== | ==Overview== | ||
| Line 10: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
1XNJ is a [ | 1XNJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XNJ OCA]. | ||
==Reference== | ==Reference== | ||
The crystal structure of human PAPS synthetase 1 reveals asymmetry in substrate binding., Harjes S, Bayer P, Scheidig AJ, J Mol Biol. 2005 Apr 1;347(3):623-35. Epub 2005 Jan 26. PMID:[http:// | The crystal structure of human PAPS synthetase 1 reveals asymmetry in substrate binding., Harjes S, Bayer P, Scheidig AJ, J Mol Biol. 2005 Apr 1;347(3):623-35. Epub 2005 Jan 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15755455 15755455] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 23: | Line 32: | ||
[[Category: aps kinase]] | [[Category: aps kinase]] | ||
[[Category: atp sulfurylase]] | [[Category: atp sulfurylase]] | ||
[[Category: | [[Category: pap]] | ||
[[Category: phosphoadenosine phosphosulfate]] | [[Category: phosphoadenosine phosphosulfate]] | ||
[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:12:13 2008'' | ||
Revision as of 16:12, 20 March 2008
| |||||||
| , resolution 1.98Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Gene: | PAPSS1, PAPSS, ATPSK1 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
APS complex of human PAPS synthetase 1
OverviewOverview
The high energy sulfate donor 3'-phosphoadenosine-5-phosphosulfate (PAPS) is used for sulfate conjugation of extracellular matrix, hormones and drugs. Human PAPS synthetase 1 catalyzes two subsequent reactions starting from ATP and sulfate. First the ATP sulfurylase domain forms APS, then the APS kinase domain phosphorylates the APS intermediate to PAPS. Up to now the interaction between the two enzymatic activities remained elusive, mainly because of missing structural information. Here we present the crystal structure of human PAPSS1 at 1.8 angstroms resolution. The structure reveals a homodimeric, asymmetric complex with the shape of a chair. The two kinase domains adopt different conformational states, with only one being able to bind its two substrates. The asymmetric binding of ADP to the APS kinase is not only observed in the crystal structure, but can also be detected in solution, using an enzymatic assay. These observations strongly indicate structural changes during the reaction cycle. Furthermore crystals soaked with ADP and APS could be prepared and the corresponding structures could be solved.
DiseaseDisease
Known disease associated with this structure: SEMD, Pakistani type OMIM:[603005]
About this StructureAbout this Structure
1XNJ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
The crystal structure of human PAPS synthetase 1 reveals asymmetry in substrate binding., Harjes S, Bayer P, Scheidig AJ, J Mol Biol. 2005 Apr 1;347(3):623-35. Epub 2005 Jan 26. PMID:15755455
Page seeded by OCA on Thu Mar 20 15:12:13 2008