1x14: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1x14.gif|left|200px]] | [[Image:1x14.gif|left|200px]] | ||
'''Crystal structure of E. coli transhydrogenase domain I with bound NAD''' | {{Structure | ||
|PDB= 1x14 |SIZE=350|CAPTION= <scene name='initialview01'>1x14</scene>, resolution 1.94Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/NAD(P)(+)_transhydrogenase_(AB-specific) NAD(P)(+) transhydrogenase (AB-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.1.2 1.6.1.2] | |||
|GENE= pntA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |||
}} | |||
'''Crystal structure of E. coli transhydrogenase domain I with bound NAD''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1X14 is a [ | 1X14 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X14 OCA]. | ||
==Reference== | ==Reference== | ||
X-ray structure of domain I of the proton-pumping membrane protein transhydrogenase from Escherichia coli., Johansson T, Oswald C, Pedersen A, Tornroth S, Okvist M, Karlsson BG, Rydstrom J, Krengel U, J Mol Biol. 2005 Sep 16;352(2):299-312. PMID:[http:// | X-ray structure of domain I of the proton-pumping membrane protein transhydrogenase from Escherichia coli., Johansson T, Oswald C, Pedersen A, Tornroth S, Okvist M, Karlsson BG, Rydstrom J, Krengel U, J Mol Biol. 2005 Sep 16;352(2):299-312. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16083909 16083909] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: NAD(P)(+) transhydrogenase (AB-specific)]] | [[Category: NAD(P)(+) transhydrogenase (AB-specific)]] | ||
| Line 27: | Line 36: | ||
[[Category: transhydrogenase]] | [[Category: transhydrogenase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:03:54 2008'' | ||
Revision as of 16:03, 20 March 2008
| |||||||
| , resolution 1.94Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | pntA (Escherichia coli) | ||||||
| Activity: | NAD(P)(+) transhydrogenase (AB-specific), with EC number 1.6.1.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of E. coli transhydrogenase domain I with bound NAD
OverviewOverview
The dimeric integral membrane protein nicotinamide nucleotide transhydrogenase is required for cellular regeneration of NADPH in mitochondria and prokaryotes, for detoxification and biosynthesis purposes. Under physiological conditions, transhydrogenase couples the reversible reduction of NADP+ by NADH to an inward proton translocation across the membrane. Here, we present crystal structures of the NAD(H)-binding domain I of transhydrogenase from Escherichia coli, in the absence as well as in the presence of oxidized and reduced substrate. The structures were determined at 1.9-2.0 A resolution. Overall, the structures are highly similar to the crystal structure of a previously published NAD(H)-binding domain, from Rhodospirillum rubrum transhydrogenase. However, this particular domain is unique, since it is covalently connected to the integral-membrane part of transhydrogenase. Comparative studies between the structures of the two species reveal extensively differing surface properties and point to the possible importance of a rigid peptide (PAPP) in the connecting linker for conformational coupling. Further, the kinetic analysis of a deletion mutant, from which the protruding beta-hairpin was removed, indicates that this structural element is important for catalytic activity, but not for domain I:domain III interaction or dimer formation. Taken together, these results have important implications for the enzyme mechanism of the large group of transhydrogenases, including mammalian enzymes, which contain a connecting linker between domains I and II.
About this StructureAbout this Structure
1X14 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
X-ray structure of domain I of the proton-pumping membrane protein transhydrogenase from Escherichia coli., Johansson T, Oswald C, Pedersen A, Tornroth S, Okvist M, Karlsson BG, Rydstrom J, Krengel U, J Mol Biol. 2005 Sep 16;352(2):299-312. PMID:16083909
Page seeded by OCA on Thu Mar 20 15:03:54 2008