1wr0: Difference between revisions

Revision as of 16:00, 20 March 2008

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Structural characterization of the MIT domain from human Vps4b

OverviewOverview

The microtubule interacting and trafficking (MIT) domain is a small protein module of unknown function that is conserved in proteins of diverse function, such as Vps4, sorting nexin 15 (SNX15), and spastin. One non-synonymous single nucleotide polymorphism was reported, which results in a Ile58-to-Met (I58M) substitution in hVps4b. Here, we have determined the solution structure of the MIT domain isolated from the NH(2)-terminus of human Vps4b, an AAA-ATPase involved in multivesicular body formation. The MIT domain adopts an 'up-and-down' three-helix bundle. Comparison with the sequences of other MIT domains clearly shows that the residues involved in inter-helical contacts are well conserved. The Ile58-to-Met substitution resulted a substantial thermal instability. In addition, we found a shallow crevice between helices A and C that may serve as a protein-binding site. We propose that the MIT domain serves as a putative adaptor domain for the ESCRT-III complex involved in endosomal trafficking.

About this StructureAbout this Structure

1WR0 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structural characterization of the MIT domain from human Vps4b., Takasu H, Jee JG, Ohno A, Goda N, Fujiwara K, Tochio H, Shirakawa M, Hiroaki H, Biochem Biophys Res Commun. 2005 Aug 26;334(2):460-5. PMID:16018968

Page seeded by OCA on Thu Mar 20 15:00:29 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1wr0.gif|left|200px]]<br /><applet load="1wr0" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1wr0.gif|left|200px]]
-caption="1wr0" />
-'''Structural characterization of the MIT domain from human Vps4b'''<br />
+ {{Structure
+|PDB= 1wr0 |SIZE=350|CAPTION= <scene name='initialview01'>1wr0</scene>
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''Structural characterization of the MIT domain from human Vps4b'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-WR0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WR0 OCA].
+WR0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WR0 OCA].
 ==Reference==
-Structural characterization of the MIT domain from human Vps4b., Takasu H, Jee JG, Ohno A, Goda N, Fujiwara K, Tochio H, Shirakawa M, Hiroaki H, Biochem Biophys Res Commun. 2005 Aug 26;334(2):460-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16018968 16018968]
+Structural characterization of the MIT domain from human Vps4b., Takasu H, Jee JG, Ohno A, Goda N, Fujiwara K, Tochio H, Shirakawa M, Hiroaki H, Biochem Biophys Res Commun. 2005 Aug 26;334(2):460-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16018968 16018968]
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
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 [[Category: mit domain]]
 [[Category: mvb]]
-[[Category: national project on protein structural and functional analyses]]
+[[Category: national project on protein structural and functional analyse]]
 [[Category: nppsfa]]
 [[Category: protein transport]]
@@ Line 31: / Line 40: @@
 [[Category: rsgi]]
 [[Category: skd1]]
-[[Category: snps]]
+[[Category: snp]]
-[[Category: structural genomics]]
+[[Category: structural genomic]]
 [[Category: vps4b]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:47:17 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:00:29 2008''