1w0d: Difference between revisions

← Older edit Newer edit →

Revision as of 15:50, 20 March 2008

File:1w0d.gif

, resolution 1.65Å

Ligands:

Activity:

3-isopropylmalate dehydrogenase, with EC number 1.1.1.85

Coordinates:

save as pdb, mmCIF, xml

THE HIGH RESOLUTION STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS LEUB (RV2995C)

OverviewOverview

The crystal structure of the enzyme 3-isopropylmalate dehydrogenase (IPMDH) from Mycobacterium tuberculosis (LeuB, Mtb-IPMDH, Rv2995c) without substrate or co-factor was determined at 1.65 A resolution, which is the highest resolution reported for an IPMDH to date. The crystals contain two functional dimers in the asymmetric unit in an arrangement close to a tetramer of D2 symmetry. Despite the absence of a substrate or inhibitor bound to the protein, the structure of the monomer resembles the previously observed closed form of the enzyme more closely than the open form. A comparison with the substrate complex of IPMDH from Thiobacillus ferrooxidans and the co-factor complex of the Thermus thermophilus enzyme revealed a close relationship of the active-site architecture between the various bacterial enzymes. The inhibitor O-isobutenyl oxalylhydroxamate was found to bind to the active site of IPMDH in a mode similar to the substrate isopropylmalate.

About this StructureAbout this Structure

1W0D is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

ReferenceReference

The high-resolution Structure of LeuB (Rv2995c) from Mycobacterium tuberculosis., Singh RK, Kefala G, Janowski R, Mueller-Dieckmann C, von Kries JP, Weiss MS, J Mol Biol. 2005 Feb 11;346(1):1-11. Epub 2004 Dec 23. PMID:15663922

Page seeded by OCA on Thu Mar 20 14:50:26 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1w0d.gif|left|200px]]<br /><applet load="1w0d" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1w0d.gif|left|200px]]
-caption="1w0d, resolution 1.65&Aring;" />
-'''THE HIGH RESOLUTION STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS LEUB (RV2995C)'''<br />
+ {{Structure
+|PDB= 1w0d |SIZE=350|CAPTION= <scene name='initialview01'>1w0d</scene>, resolution 1.65&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/3-isopropylmalate_dehydrogenase 3-isopropylmalate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.85 1.1.1.85]
+|GENE=
+}}
+'''THE HIGH RESOLUTION STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS LEUB (RV2995C)'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-W0D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/3-isopropylmalate_dehydrogenase 3-isopropylmalate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.85 1.1.1.85] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W0D OCA].
+W0D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W0D OCA].
 ==Reference==
-The high-resolution Structure of LeuB (Rv2995c) from Mycobacterium tuberculosis., Singh RK, Kefala G, Janowski R, Mueller-Dieckmann C, von Kries JP, Weiss MS, J Mol Biol. 2005 Feb 11;346(1):1-11. Epub 2004 Dec 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15663922 15663922]
+The high-resolution Structure of LeuB (Rv2995c) from Mycobacterium tuberculosis., Singh RK, Kefala G, Janowski R, Mueller-Dieckmann C, von Kries JP, Weiss MS, J Mol Biol. 2005 Feb 11;346(1):1-11. Epub 2004 Dec 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15663922 15663922]
 [[Category: 3-isopropylmalate dehydrogenase]]
 [[Category: Mycobacterium tuberculosis]]
@@ Line 27: / Line 36: @@
 [[Category: protein structure initiative]]
 [[Category: psi]]
-[[Category: structural genomics]]
+[[Category: structural genomic]]
 [[Category: tb]]
 [[Category: tb structural genomics consortium]]
 [[Category: tbsgc]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:39:09 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:50:26 2008''