1v9f: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1v9f.jpg|left|200px]] | [[Image:1v9f.jpg|left|200px]] | ||
'''Crystal structure of catalytic domain of pseudouridine synthase RluD from Escherichia coli''' | {{Structure | ||
|PDB= 1v9f |SIZE=350|CAPTION= <scene name='initialview01'>1v9f</scene>, resolution 1.70Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Pseudouridylate_synthase Pseudouridylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.70 4.2.1.70] | |||
|GENE= RLUD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |||
}} | |||
'''Crystal structure of catalytic domain of pseudouridine synthase RluD from Escherichia coli''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1V9F is a [ | 1V9F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V9F OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structures of the catalytic domains of pseudouridine synthases RluC and RluD from Escherichia coli., Mizutani K, Machida Y, Unzai S, Park SY, Tame JR, Biochemistry. 2004 Apr 20;43(15):4454-63. PMID:[http:// | Crystal structures of the catalytic domains of pseudouridine synthases RluC and RluD from Escherichia coli., Mizutani K, Machida Y, Unzai S, Park SY, Tame JR, Biochemistry. 2004 Apr 20;43(15):4454-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15078091 15078091] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Pseudouridylate synthase]] | [[Category: Pseudouridylate synthase]] | ||
| Line 23: | Line 32: | ||
[[Category: rna binding]] | [[Category: rna binding]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:42:35 2008'' | ||
Revision as of 15:42, 20 March 2008
| |||||||
| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | RLUD (Escherichia coli) | ||||||
| Activity: | Pseudouridylate synthase, with EC number 4.2.1.70 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of catalytic domain of pseudouridine synthase RluD from Escherichia coli
OverviewOverview
The most frequent modification of RNA, the conversion of uridine bases to pseudouridines, is found in all living organisms and often in highly conserved locations in ribosomal and transfer RNA. RluC and RluD are homologous enzymes which each convert three specific uridine bases in Escherichia coli ribosomal 23S RNA to pseudouridine: bases 955, 2504, and 2580 in the case of RluC and 1911, 1915, and 1917 in the case of RluD. Both have an N-terminal S4 RNA binding domain. While the loss of RluC has little phenotypic effect, loss of RluD results in a much reduced growth rate. We have determined the crystal structures of the catalytic domain of RluC, and full-length RluD. The S4 domain of RluD appears to be highly flexible or unfolded and is completely invisible in the electron density map. Despite the conserved topology shared by the two proteins, the surface shape and charge distribution are very different. The models suggest significant differences in substrate binding by different pseudouridine synthases.
About this StructureAbout this Structure
1V9F is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structures of the catalytic domains of pseudouridine synthases RluC and RluD from Escherichia coli., Mizutani K, Machida Y, Unzai S, Park SY, Tame JR, Biochemistry. 2004 Apr 20;43(15):4454-63. PMID:15078091
Page seeded by OCA on Thu Mar 20 14:42:35 2008