1v9p: Difference between revisions
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[[Image:1v9p.gif|left|200px]] | [[Image:1v9p.gif|left|200px]] | ||
'''Crystal Structure Of Nad+-Dependent DNA Ligase''' | {{Structure | ||
|PDB= 1v9p |SIZE=350|CAPTION= <scene name='initialview01'>1v9p</scene>, resolution 2.90Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=AMP:ADENOSINE MONOPHOSPHATE'>AMP</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/DNA_ligase_(NAD(+)) DNA ligase (NAD(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.2 6.5.1.2] | |||
|GENE= LIGA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=276 Thermus filiformis]) | |||
}} | |||
'''Crystal Structure Of Nad+-Dependent DNA Ligase''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1V9P is a [ | 1V9P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_filiformis Thermus filiformis]. This structure supersedes the now removed PDB entry 1DGT. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V9P OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of NAD(+)-dependent DNA ligase: modular architecture and functional implications., Lee JY, Chang C, Song HK, Moon J, Yang JK, Kim HK, Kwon ST, Suh SW, EMBO J. 2000 Mar 1;19(5):1119-29. PMID:[http:// | Crystal structure of NAD(+)-dependent DNA ligase: modular architecture and functional implications., Lee JY, Chang C, Song HK, Moon J, Yang JK, Kim HK, Kwon ST, Suh SW, EMBO J. 2000 Mar 1;19(5):1119-29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10698952 10698952] | ||
[[Category: DNA ligase (NAD(+))]] | [[Category: DNA ligase (NAD(+))]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: nad+-dependent dna ligase]] | [[Category: nad+-dependent dna ligase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:42:41 2008'' | ||
Revision as of 15:42, 20 March 2008
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| , resolution 2.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | LIGA (Thermus filiformis) | ||||||
| Activity: | DNA ligase (NAD(+)), with EC number 6.5.1.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure Of Nad+-Dependent DNA Ligase
OverviewOverview
DNA ligases catalyze the crucial step of joining the breaks in duplex DNA during DNA replication, repair and recombination, utilizing either ATP or NAD(+) as a cofactor. Despite the difference in cofactor specificity and limited overall sequence similarity, the two classes of DNA ligase share basically the same catalytic mechanism. In this study, the crystal structure of an NAD(+)-dependent DNA ligase from Thermus filiformis, a 667 residue multidomain protein, has been determined by the multiwavelength anomalous diffraction (MAD) method. It reveals highly modular architecture and a unique circular arrangement of its four distinct domains. It also provides clues for protein flexibility and DNA-binding sites. A model for the multidomain ligase action involving large conformational changes is proposed.
About this StructureAbout this Structure
1V9P is a Single protein structure of sequence from Thermus filiformis. This structure supersedes the now removed PDB entry 1DGT. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of NAD(+)-dependent DNA ligase: modular architecture and functional implications., Lee JY, Chang C, Song HK, Moon J, Yang JK, Kim HK, Kwon ST, Suh SW, EMBO J. 2000 Mar 1;19(5):1119-29. PMID:10698952
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