1uz6: Difference between revisions

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ANTI-LEWIS X FAB FRAGMENT UNCOMPLEXED

OverviewOverview

The Lewis X trisaccharide is pivotal in mediating specific cell-cell interactions. Monoclonal antibody 291-2G3-A, which was generated from mice infected with schistosomes, has been shown to recognize the Lewis X trisaccharide. Here we describe the structure of the Fab fragment of 291-2G3-A, with Lewis X, to 1.8 A resolution. The crystallographic analysis revealed that the antigen binding site is a rather shallow binding pocket, and residues from all six complementary determining regions of the antibody contact all sugar residues. The high specificity of the binding pocket does not result in high affinity; the K(D) determined by isothermal calorimetry is 11 microM. However, this affinity is in the same range as for other sugar-antibody complexes. The detailed understanding of the antibody-Lewis X interaction revealed by the crystal structure may be helpful in the design of better diagnostic tools for schistosomiasis and for studying Lewis X-mediated cell-cell interactions by antibody interference.

About this StructureAbout this Structure

1UZ6 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Structure of an anti-Lewis X Fab fragment in complex with its Lewis X antigen., van Roon AM, Pannu NS, de Vrind JP, van der Marel GA, van Boom JH, Hokke CH, Deelder AM, Abrahams JP, Structure. 2004 Jul;12(7):1227-36. PMID:15242599

Page seeded by OCA on Thu Mar 20 14:38:50 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1uz6.jpg|left|200px]]<br /><applet load="1uz6" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1uz6.jpg|left|200px]]
-caption="1uz6, resolution 2.05&Aring;" />
-'''ANTI-LEWIS X FAB FRAGMENT UNCOMPLEXED'''<br />
+ {{Structure
+|PDB= 1uz6 |SIZE=350|CAPTION= <scene name='initialview01'>1uz6</scene>, resolution 2.05&Aring;
+|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+V'>AC1</scene>
+|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''ANTI-LEWIS X FAB FRAGMENT UNCOMPLEXED'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-UZ6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+V'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UZ6 OCA].
+UZ6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UZ6 OCA].
 ==Reference==
-Structure of an anti-Lewis X Fab fragment in complex with its Lewis X antigen., van Roon AM, Pannu NS, de Vrind JP, van der Marel GA, van Boom JH, Hokke CH, Deelder AM, Abrahams JP, Structure. 2004 Jul;12(7):1227-36. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15242599 15242599]
+Structure of an anti-Lewis X Fab fragment in complex with its Lewis X antigen., van Roon AM, Pannu NS, de Vrind JP, van der Marel GA, van Boom JH, Hokke CH, Deelder AM, Abrahams JP, Structure. 2004 Jul;12(7):1227-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15242599 15242599]
 [[Category: Mus musculus]]
 [[Category: Single protein]]
@@ Line 25: / Line 34: @@
 [[Category: antibody]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:29:57 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:38:50 2008''