1utb: Difference between revisions
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[[Image:1utb.gif|left|200px]] | [[Image:1utb.gif|left|200px]] | ||
'''DNTR FROM BURKHOLDERIA SP. STRAIN DNT''' | {{Structure | ||
|PDB= 1utb |SIZE=350|CAPTION= <scene name='initialview01'>1utb</scene>, resolution 2.59Å | |||
|SITE= <scene name='pdbsite=AC1:Act+Binding+Site+For+Chain+B'>AC1</scene> | |||
|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''DNTR FROM BURKHOLDERIA SP. STRAIN DNT''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1UTB is a [ | 1UTB is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Burkholderia_sp. Burkholderia sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UTB OCA]. | ||
==Reference== | ==Reference== | ||
Development of a bacterial biosensor for nitrotoluenes: the crystal structure of the transcriptional regulator DntR., Smirnova IA, Dian C, Leonard GA, McSweeney S, Birse D, Brzezinski P, J Mol Biol. 2004 Jul 9;340(3):405-18. PMID:[http:// | Development of a bacterial biosensor for nitrotoluenes: the crystal structure of the transcriptional regulator DntR., Smirnova IA, Dian C, Leonard GA, McSweeney S, Birse D, Brzezinski P, J Mol Biol. 2004 Jul 9;340(3):405-18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15210343 15210343] | ||
[[Category: Burkholderia sp.]] | [[Category: Burkholderia sp.]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: transcriptional regulator]] | [[Category: transcriptional regulator]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:36:30 2008'' | ||
Revision as of 15:36, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
DNTR FROM BURKHOLDERIA SP. STRAIN DNT
OverviewOverview
The transcriptional regulator DntR, a member of the LysR family, is a central element in a prototype bacterial cell-based biosensor for the detection of hazardous contamination of soil and groundwater by dinitrotoluenes. To optimise the sensitivity of the biosensor for such compounds we have chosen a rational design of the inducer-binding cavity based on knowledge of the three-dimensional structure of DntR. We report two crystal structures of DntR with acetate (resolution 2.6 angstroms) and thiocyanate (resolution 2.3 angstroms), respectively, occupying the inducer-binding cavity. These structures allow for the construction of models of DntR in complex with salicylate (Kd approximately or = 4 microM) and 2,4-dinitrotoluene that provide a basis for the design of mutant DntR with enhanced specificity for dinitrotoluenes. In both crystal structures DntR crystallises as a homodimer with a "head-to-tail" arrangement of monomers in the asymmetric unit. Analysis of the crystal structure has allowed the building of a full-length model of DntR in its biologically active homotetrameric form consisting of two "head-to-head" dimers. The implications of this model for the mechanism of transcription regulation by LysR proteins are discussed.
About this StructureAbout this Structure
1UTB is a Protein complex structure of sequences from Burkholderia sp.. Full crystallographic information is available from OCA.
ReferenceReference
Development of a bacterial biosensor for nitrotoluenes: the crystal structure of the transcriptional regulator DntR., Smirnova IA, Dian C, Leonard GA, McSweeney S, Birse D, Brzezinski P, J Mol Biol. 2004 Jul 9;340(3):405-18. PMID:15210343
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