1usx: Difference between revisions
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[[Image:1usx.gif|left|200px]] | [[Image:1usx.gif|left|200px]] | ||
'''CRYSTAL STRUCTURE OF THE NEWCASTLE DISEASE VIRUS HEMAGGLUTININ-NEURAMINIDASE COMPLEXED WITH THIOSIALOSIDE''' | {{Structure | ||
|PDB= 1usx |SIZE=350|CAPTION= <scene name='initialview01'>1usx</scene>, resolution 2.7Å | |||
|SITE= <scene name='pdbsite=AC1:Dan+Binding+Site+For+Chain+C'>AC1</scene> | |||
|LIGAND= <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene>, <scene name='pdbligand=DAN:2-DEOXY-2,3-DEHYDRO-N-ACETYL-NEURAMINIC+ACID'>DAN</scene> and <scene name='pdbligand=WIA:METHYL(6S)-1-THIO-L-MANNO-HEXODIALDO-6,2-PYRANOSIDE'>WIA</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] | |||
|GENE= | |||
}} | |||
'''CRYSTAL STRUCTURE OF THE NEWCASTLE DISEASE VIRUS HEMAGGLUTININ-NEURAMINIDASE COMPLEXED WITH THIOSIALOSIDE''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1USX is a [ | 1USX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Newcastle_disease_virus Newcastle disease virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1USX OCA]. | ||
==Reference== | ==Reference== | ||
Second sialic acid binding site in Newcastle disease virus hemagglutinin-neuraminidase: implications for fusion., Zaitsev V, von Itzstein M, Groves D, Kiefel M, Takimoto T, Portner A, Taylor G, J Virol. 2004 Apr;78(7):3733-41. PMID:[http:// | Second sialic acid binding site in Newcastle disease virus hemagglutinin-neuraminidase: implications for fusion., Zaitsev V, von Itzstein M, Groves D, Kiefel M, Takimoto T, Portner A, Taylor G, J Virol. 2004 Apr;78(7):3733-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15016893 15016893] | ||
[[Category: Exo-alpha-sialidase]] | [[Category: Exo-alpha-sialidase]] | ||
[[Category: Newcastle disease virus]] | [[Category: Newcastle disease virus]] | ||
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[[Category: sialidase]] | [[Category: sialidase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:36:15 2008'' | ||
Revision as of 15:36, 20 March 2008
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| , resolution 2.7Å | |||||||
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| Sites: | |||||||
| Ligands: | , and | ||||||
| Activity: | Exo-alpha-sialidase, with EC number 3.2.1.18 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF THE NEWCASTLE DISEASE VIRUS HEMAGGLUTININ-NEURAMINIDASE COMPLEXED WITH THIOSIALOSIDE
OverviewOverview
Paramyxoviruses are the leading cause of respiratory disease in children. Several paramyxoviruses possess a surface glycoprotein, the hemagglutinin-neuraminidase (HN), that is involved in attachment to sialic acid receptors, promotion of fusion, and removal of sialic acid from infected cells and progeny virions. Previously we showed that Newcastle disease virus (NDV) HN contained a pliable sialic acid recognition site that could take two states, a binding state and a catalytic state. Here we present evidence for a second sialic acid binding site at the dimer interface of HN and present a model for its involvement in cell fusion. Three different crystal forms of NDV HN now reveal identical tetrameric arrangements of HN monomers, perhaps indicative of the tetramer association found on the viral surface.
About this StructureAbout this Structure
1USX is a Single protein structure of sequence from Newcastle disease virus. Full crystallographic information is available from OCA.
ReferenceReference
Second sialic acid binding site in Newcastle disease virus hemagglutinin-neuraminidase: implications for fusion., Zaitsev V, von Itzstein M, Groves D, Kiefel M, Takimoto T, Portner A, Taylor G, J Virol. 2004 Apr;78(7):3733-41. PMID:15016893
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