1u7c: Difference between revisions

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Revision as of 15:28, 20 March 2008

File:1u7c.jpg

, resolution 1.85Å

Ligands:

Gene:

amtB (Escherichia coli)

Coordinates:

save as pdb, mmCIF, xml

Crystal Structure of AmtB from E.Coli with Methyl Ammonium.

OverviewOverview

The first structure of an ammonia channel from the Amt/MEP/Rh protein superfamily, determined to 1.35 angstrom resolution, shows it to be a channel that spans the membrane 11 times. Two structurally similar halves span the membrane with opposite polarity. Structures with and without ammonia or methyl ammonia show a vestibule that recruits NH4+/NH3, a binding site for NH4+, and a 20 angstrom-long hydrophobic channel that lowers the NH4+ pKa to below 6 and conducts NH3. Favorable interactions for NH3 are seen within the channel and use conserved histidines. Reconstitution of AmtB into vesicles shows that AmtB conducts uncharged NH3.

About this StructureAbout this Structure

1U7C is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A., Khademi S, O'Connell J 3rd, Remis J, Robles-Colmenares Y, Miercke LJ, Stroud RM, Science. 2004 Sep 10;305(5690):1587-94. PMID:15361618

Page seeded by OCA on Thu Mar 20 14:28:13 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1u7c.jpg|left|200px]]<br /><applet load="1u7c" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1u7c.jpg|left|200px]]
-caption="1u7c, resolution 1.85&Aring;" />
-'''Crystal Structure of AmtB from E.Coli with Methyl Ammonium.'''<br />
+ {{Structure
+|PDB= 1u7c |SIZE=350|CAPTION= <scene name='initialview01'>1u7c</scene>, resolution 1.85&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=NME:METHYLAMINE'>NME</scene>
+|ACTIVITY=
+|GENE= amtB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+}}
+'''Crystal Structure of AmtB from E.Coli with Methyl Ammonium.'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-U7C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=NME:'>NME</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U7C OCA].
+U7C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U7C OCA].
 ==Reference==
-Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A., Khademi S, O'Connell J 3rd, Remis J, Robles-Colmenares Y, Miercke LJ, Stroud RM, Science. 2004 Sep 10;305(5690):1587-94. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15361618 15361618]
+Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A., Khademi S, O'Connell J 3rd, Remis J, Robles-Colmenares Y, Miercke LJ, Stroud RM, Science. 2004 Sep 10;305(5690):1587-94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15361618 15361618]
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
@@ Line 24: / Line 33: @@
 [[Category: methy ammonium]]
 [[Category: right handed helical bundle]]
-[[Category: transmembrane helices]]
+[[Category: transmembrane helice]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:21:19 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:28:13 2008''