1u0b: Difference between revisions
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'''Crystal structure of cysteinyl-tRNA synthetase binary complex with tRNACys''' | {{Structure | ||
|PDB= 1u0b |SIZE=350|CAPTION= <scene name='initialview01'>1u0b</scene>, resolution 2.30Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Cysteine--tRNA_ligase Cysteine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.16 6.1.1.16] | |||
|GENE= cysS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |||
}} | |||
'''Crystal structure of cysteinyl-tRNA synthetase binary complex with tRNACys''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1U0B is a [ | 1U0B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0B OCA]. | ||
==Reference== | ==Reference== | ||
Shape-selective RNA recognition by cysteinyl-tRNA synthetase., Hauenstein S, Zhang CM, Hou YM, Perona JJ, Nat Struct Mol Biol. 2004 Nov;11(11):1134-41. Epub 2004 Oct 17. PMID:[http:// | Shape-selective RNA recognition by cysteinyl-tRNA synthetase., Hauenstein S, Zhang CM, Hou YM, Perona JJ, Nat Struct Mol Biol. 2004 Nov;11(11):1134-41. Epub 2004 Oct 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15489861 15489861] | ||
[[Category: Cysteine--tRNA ligase]] | [[Category: Cysteine--tRNA ligase]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
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[[Category: protein-rna complex]] | [[Category: protein-rna complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:25:35 2008'' | ||
Revision as of 15:25, 20 March 2008
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| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | cysS (Escherichia coli) | ||||||
| Activity: | Cysteine--tRNA ligase, with EC number 6.1.1.16 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of cysteinyl-tRNA synthetase binary complex with tRNACys
OverviewOverview
The crystal structure of Escherichia coli cysteinyl-tRNA synthetase (CysRS) bound to tRNA(Cys) at a resolution of 2.3 A reveals base-specific and shape-selective interactions across an extensive protein-RNA recognition interface. The complex contains a mixed alpha/beta C-terminal domain, which is disordered in the unliganded enzyme. This domain makes specific hydrogen bonding interactions with all three bases of the GCA anticodon. The tRNA anticodon stem is bent sharply toward the enzyme as compared with its conformation when bound to elongation factor Tu, providing an essential basis for shape-selective recognition. The CysRS structure also reveals interactions of conserved enzyme groups with the sugar-phosphate backbone in the D loop, adjacent to an unusual G15.G48 tertiary base pair previously implicated in tRNA aminoacylation. A combined mutational analysis of enzyme and tRNA groups at G15.G48 supports the notion that contacts between CysRS and the sugar-phosphate backbone contribute to recognition by indirect readout.
About this StructureAbout this Structure
1U0B is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Shape-selective RNA recognition by cysteinyl-tRNA synthetase., Hauenstein S, Zhang CM, Hou YM, Perona JJ, Nat Struct Mol Biol. 2004 Nov;11(11):1134-41. Epub 2004 Oct 17. PMID:15489861
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