Sandbox Reserved 718: Difference between revisions

Let-7 is a key target of Lin28 but there are four additional functions:  

First, during retinoic-acid-induced neurogliogenesis, Lin28a overexpression altered the expression of several transcription factors involved in early embryonic cell fate decision before any increase in let-7 level was detected. <ref>Balzer, E., Heine, C., Jiang, Q., Lee, V.M., and Moss, E.G. In: ''LIN28 alters cell fate succession and acts independently of the let-7 microRNA during neurogliogenesis in vitro. Development 137, S.891–900, 2010.</ref> Second, impaired glucose tolerance and insulin resistance were observed in muscle-specific Lin28a knockout mice without significant changes in the let-7 level.<ref>Zhu, H., Shyh-Chang, N., Segre , A.V., Shinoda, G., Shah, S.P., Einhorn, W.S., Takeuchi, A., Engreitz, J.M., Hagan, J.P., Kharas, M.G., et al; DIAGRAM Consortium; MAGIC Investigators. In: ''The Lin28/let-7 axis regulates glucose metabolism.'' Cell 147, S.81–94, 2011.</ref> Third, Lin28A interacts with mRNAs and cosediments with polysome in sucrose gradient centrifugation.<ref>Erica Balzer and Eric G. Moss: ''Localization of the Developmental Timing Regulator Lin28 to mRNP Complexes, P-bodies and Stress Granules.'' In: RNA Biology 4:1, S.16-25, January/February/March 2007.</ref> Consistently, several studies reported that Lin28A can bind to and enhance translation of certain mRNAs such as Igf2 in differentiating myoblasts and Oct4 in ESCs.<ref>Polesskaya, A., Cuvellier, S., Naguibneva, I., Duquet, A., Moss, E.G., and Harel-Bellan, A. In: ''Lin-28 binds IGF-2 mRNA and participates in skeletal myogenesis by increasing translation efficiency.'' Genes Dev. 21, 1125–1138, 2007.</ref><ref>Qiu, C., Ma, Y., Wang, J., Peng, S., and Huang, Y. In: ''Lin28-mediated post-transcriptional regulation of Oct4 expression in human embryonic stem cells.'' Nucleic Acids Res. 38, S.1240–1248, 2010.</ref><ref>Xu, B., and Huang, Y. In: ''Histone H2a mRNA interacts with Lin28 and contains a Lin28-dependent posttranscriptional regulatory element.'' Nucleic Acids Res. 37, S.4256–4263, 2009.</ref><ref>Xu, B., Zhang, K., and Huang, Y. In: ''Lin28 modulates cell growth and associates with a subset of cell cycle regulator mRNAs in mouse embryonic stem cells.'' RNA 15, S.357–361, 2009.</ref>

== '''Applications''' ==