TAL effector: Difference between revisions

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OCA, Philipp Warmer, Michal Harel, Jaime Prilusky, Alexander Berchansky

Revision as of 15:02, 3 January 2013 view source Philipp Warmer (talk \| contribs) 100 edits No edit summary ← Older edit		Revision as of 15:06, 3 January 2013 view source Philipp Warmer (talk \| contribs) 100 edits No edit summary Newer edit →
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	<br>[[Image:sanjana.png\|left\|400px\|thumb\|Fig.2]]<br>		<br>[[Image:sanjana.png\|left\|400px\|thumb\|Fig.2]]<br>
	'''Figure 2''': Natural structure of TALEs derived from Xanthomonas sp. Each DNA-binding module consists of 34 amino acids, where the RVDs in the 12th and 13th amino acid positions of each repeat specify the DNA base being targeted according to the cipher NG = T, HD = <scene name='Sandbox_Reserved_701/C_schwarz/1'>C</scene>, NI = <scene name='Sandbox_Reserved_701/A_gruen/1'>A</scene>, and NN = <scene name='Sandbox_Reserved_701/G_gelb/1'>G</scene> or <scene name='Sandbox_Reserved_701/A_gruen/1'>A</scene>. The DNA-binding modules are flanked by nonrepetitive N and C termini, which carry the translocation, nuclear localization (NLS) and transcription activation (AD) domains. A cryptic signal within the N terminus specifies a thymine as the first base of the target site.<ref> PMID:022223736 </ref>		'''Figure 2''': Natural structure of TALEs derived from Xanthomonas sp. Each DNA-binding module consists of 34 amino acids, where the RVDs in the 12th and 13th amino acid positions of each repeat specify the DNA base being targeted according to the cipher NG = <scene name='Sandbox_Reserved_701/T_blau/1'>T</scene>, HD = <scene name='Sandbox_Reserved_701/C_schwarz/1'>C</scene>, NI = <scene name='Sandbox_Reserved_701/A_gruen/1'>A</scene>, and NN = <scene name='Sandbox_Reserved_701/G_gelb/1'>G</scene> or <scene name='Sandbox_Reserved_701/A_gruen/1'>A</scene>. The DNA-binding modules are flanked by nonrepetitive N and C termini, which carry the translocation, nuclear localization (NLS) and transcription activation (AD) domains. A cryptic signal within the N terminus specifies a thymine as the first base of the target site.<ref> PMID:022223736 </ref>