4gx2: Difference between revisions
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[[ | ==GsuK channel bound to NAD== | ||
<StructureSection load='4gx2' size='340' side='right' caption='[[4gx2]], [[Resolution|resolution]] 3.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4gx2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacter_sulfurreducens_pca Geobacter sulfurreducens pca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GX2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GX2 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4gvl|4gvl]], [[4gx0|4gx0]], [[4gx1|4gx1]], [[4gx5|4gx5]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GSU0527 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243231 Geobacter sulfurreducens PCA])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gx2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gx2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gx2 RCSB], [http://www.ebi.ac.uk/pdbsum/4gx2 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The gating ring-forming RCK domain regulates channel gating in response to various cellular chemical stimuli in eukaryotic Slo channel families and the majority of ligand-gated prokaryotic K(+) channels and transporters. Here we present structural and functional studies of a dual RCK-containing, multi-ligand gated K(+) channel from Geobacter sulfurreducens, named GsuK. We demonstrate that ADP and NAD(+) activate the GsuK channel, whereas Ca(2+) serves as an allosteric inhibitor. Multiple crystal structures elucidate the structural basis of multi-ligand gating in GsuK, and also reveal a unique ion conduction pore with segmented inner helices. Structural comparison leads us to propose a novel pore opening mechanics that is distinct from other K(+) channels.DOI:http://dx.doi.org/10.7554/eLife.00184.001. | |||
Distinct gating mechanisms revealed by the structures of a multi-ligand gated K(+) channel.,Kong C, Zeng W, Ye S, Chen L, Sauer DB, Lam Y, Derebe MG, Jiang Y elife. 2012;1:e00184. doi: 10.7554/eLife.00184. Epub 2012 Dec 13. PMID:23240087<ref>PMID:23240087</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Geobacter sulfurreducens pca]] | [[Category: Geobacter sulfurreducens pca]] | ||
[[Category: Chen, L | [[Category: Chen, L]] | ||
[[Category: Derebe, M G | [[Category: Derebe, M G]] | ||
[[Category: Jiang, Y | [[Category: Jiang, Y]] | ||
[[Category: Kong, C | [[Category: Kong, C]] | ||
[[Category: Lam, Y | [[Category: Lam, Y]] | ||
[[Category: Sauer, D B | [[Category: Sauer, D B]] | ||
[[Category: Ye, S | [[Category: Ye, S]] | ||
[[Category: Zeng, W | [[Category: Zeng, W]] | ||
[[Category: Adp binding]] | [[Category: Adp binding]] | ||
[[Category: Ion channel]] | [[Category: Ion channel]] | ||
Revision as of 12:14, 10 December 2014
GsuK channel bound to NADGsuK channel bound to NAD
Structural highlights
Publication Abstract from PubMedThe gating ring-forming RCK domain regulates channel gating in response to various cellular chemical stimuli in eukaryotic Slo channel families and the majority of ligand-gated prokaryotic K(+) channels and transporters. Here we present structural and functional studies of a dual RCK-containing, multi-ligand gated K(+) channel from Geobacter sulfurreducens, named GsuK. We demonstrate that ADP and NAD(+) activate the GsuK channel, whereas Ca(2+) serves as an allosteric inhibitor. Multiple crystal structures elucidate the structural basis of multi-ligand gating in GsuK, and also reveal a unique ion conduction pore with segmented inner helices. Structural comparison leads us to propose a novel pore opening mechanics that is distinct from other K(+) channels.DOI:http://dx.doi.org/10.7554/eLife.00184.001. Distinct gating mechanisms revealed by the structures of a multi-ligand gated K(+) channel.,Kong C, Zeng W, Ye S, Chen L, Sauer DB, Lam Y, Derebe MG, Jiang Y elife. 2012;1:e00184. doi: 10.7554/eLife.00184. Epub 2012 Dec 13. PMID:23240087[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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