Sandbox Reserved 712: Difference between revisions
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The relative vitality is related to the phenotypic changes in the susceptibility to darunavir. As one can see in the diagram, the more darunavir-associated mutations there are, the higher is the relative vitality (PR<sub>DRV4</sub> > PR<sub>DRV1</sub> > PR<sub>DRV2</sub> > PR<sub>DRV6</sub>). Due to the fact that PR<sub>DRV5</sub> does not have the key mutations, it has in comparison to the other samples a low vitality value for darunavir and the structural related amprenavir. The lopinavir pattern looks different than the overall pattern of darunavir and amprenavir, because it has a different structure and resistance profile than the others.(Fig1) <ref name="Molecular"> PMID:19535439 </ref> | The relative vitality is related to the phenotypic changes in the susceptibility to darunavir. As one can see in the diagram, the more darunavir-associated mutations there are, the higher is the relative vitality (PR<sub>DRV4</sub> > PR<sub>DRV1</sub> > PR<sub>DRV2</sub> > PR<sub>DRV6</sub>). Due to the fact that PR<sub>DRV5</sub> does not have the key mutations, it has in comparison to the other samples a low vitality value for darunavir and the structural related amprenavir. The lopinavir pattern looks different than the overall pattern of darunavir and amprenavir, because it has a different structure and resistance profile than the others.(Fig1) <ref name="Molecular"> PMID:19535439 </ref> | ||
[[Image:Relative_vitality_values_for_recombinant_PRs_and_PRIs.jpg | thumb | left | Fig.1 Relative vitality values <ref name="Molecular"/>]] | [[Image:Relative_vitality_values_for_recombinant_PRs_and_PRIs.jpg | thumb | left | Fig.1 Relative vitality values. <ref name="Molecular"/>]] | ||
Despite the many mutations the k<sub>cat</sub> values were still between 30% and 50% of the wild-type value. In contrast the K<sub>m</sub> values of the mutants were (mostly) four- to eightfold higher than the wild-type PR. | Despite the many mutations the k<sub>cat</sub> values were still between 30% and 50% of the wild-type value. In contrast the K<sub>m</sub> values of the mutants were (mostly) four- to eightfold higher than the wild-type PR. | ||