1sru: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1sru.gif|left|200px]] | [[Image:1sru.gif|left|200px]] | ||
'''Crystal structure of full length E. coli SSB protein''' | {{Structure | ||
|PDB= 1sru |SIZE=350|CAPTION= <scene name='initialview01'>1sru</scene>, resolution 3.30Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= SSB, EXRB, LEXC, B4059, C5049, Z5658, ECS5041, SF4145, S3584 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |||
}} | |||
'''Crystal structure of full length E. coli SSB protein''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1SRU is a [ | 1SRU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SRU OCA]. | ||
==Reference== | ==Reference== | ||
The C-terminal domain of full-length E. coli SSB is disordered even when bound to DNA., Savvides SN, Raghunathan S, Futterer K, Kozlov AG, Lohman TM, Waksman G, Protein Sci. 2004 Jul;13(7):1942-7. Epub 2004 May 28. PMID:[http:// | The C-terminal domain of full-length E. coli SSB is disordered even when bound to DNA., Savvides SN, Raghunathan S, Futterer K, Kozlov AG, Lohman TM, Waksman G, Protein Sci. 2004 Jul;13(7):1942-7. Epub 2004 May 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15169953 15169953] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 21: | Line 30: | ||
[[Category: replication]] | [[Category: replication]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:08:51 2008'' | ||
Revision as of 15:08, 20 March 2008
| |||||||
| , resolution 3.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | SSB, EXRB, LEXC, B4059, C5049, Z5658, ECS5041, SF4145, S3584 (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of full length E. coli SSB protein
OverviewOverview
The crystal structure of full-length homotetrameric single-stranded DNA (ssDNA)-binding protein from Escherichia coli (SSB) has been determined to 3.3 A resolution and reveals that the entire C-terminal domain is disordered even in the presence of ssDNA. To our knowledge, this is the first experimental evidence that the C-terminal domain of SSB may be inherently disordered. The N-terminal DNA-binding domain of the protein is well ordered and is virtually indistinguishable from the previously determined structure of the chymotryptic fragment of SSB (SSBc) in complex with ssDNA. The absence of observable interactions with the core protein and the crystal packing of SSB together suggest that the disordered C-terminal domains likely extend laterally away from the DNA- binding domains, which may facilitate interactions with components of the replication machinery in vivo. The structure also reveals the conservation of molecular contacts between successive tetramers mediated by the L(45) loops as seen in two other crystal forms of SSBc, suggesting a possible functional relevance of this interaction.
About this StructureAbout this Structure
1SRU is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
The C-terminal domain of full-length E. coli SSB is disordered even when bound to DNA., Savvides SN, Raghunathan S, Futterer K, Kozlov AG, Lohman TM, Waksman G, Protein Sci. 2004 Jul;13(7):1942-7. Epub 2004 May 28. PMID:15169953
Page seeded by OCA on Thu Mar 20 14:08:51 2008