1shw: Difference between revisions
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[[Image:1shw.gif|left|200px]] | [[Image:1shw.gif|left|200px]] | ||
'''EphB2 / EphrinA5 Complex Structure''' | {{Structure | ||
|PDB= 1shw |SIZE=350|CAPTION= <scene name='initialview01'>1shw</scene>, resolution 2.2Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | |||
|ACTIVITY= | |||
|GENE= EFNA5, EPLG7, LERK7, EPL7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]), EPHB2, EPTH3, NUK, SEK3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | |||
}} | |||
'''EphB2 / EphrinA5 Complex Structure''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1SHW is a [ | 1SHW is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SHW OCA]. | ||
==Reference== | ==Reference== | ||
Repelling class discrimination: ephrin-A5 binds to and activates EphB2 receptor signaling., Himanen JP, Chumley MJ, Lackmann M, Li C, Barton WA, Jeffrey PD, Vearing C, Geleick D, Feldheim DA, Boyd AW, Henkemeyer M, Nikolov DB, Nat Neurosci. 2004 May;7(5):501-9. Epub 2004 Apr 25. PMID:[http:// | Repelling class discrimination: ephrin-A5 binds to and activates EphB2 receptor signaling., Himanen JP, Chumley MJ, Lackmann M, Li C, Barton WA, Jeffrey PD, Vearing C, Geleick D, Feldheim DA, Boyd AW, Henkemeyer M, Nikolov DB, Nat Neurosci. 2004 May;7(5):501-9. Epub 2004 Apr 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15107857 15107857] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: receptor tyrosine kinase]] | [[Category: receptor tyrosine kinase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:05:10 2008'' | ||
Revision as of 15:05, 20 March 2008
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| , resolution 2.2Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | EFNA5, EPLG7, LERK7, EPL7 (Mus musculus), EPHB2, EPTH3, NUK, SEK3 (Mus musculus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
EphB2 / EphrinA5 Complex Structure
OverviewOverview
The interactions between Eph receptor tyrosine kinases and their ephrin ligands regulate cell migration and axon pathfinding. The EphA receptors are generally thought to become activated by ephrin-A ligands, whereas the EphB receptors interact with ephrin-B ligands. Here we show that two of the most widely studied of these molecules, EphB2 and ephrin-A5, which have never been described to interact with each other, do in fact bind one another with high affinity. Exposure of EphB2-expressing cells to ephrin-A5 leads to receptor clustering, autophosphorylation and initiation of downstream signaling. Ephrin-A5 induces EphB2-mediated growth cone collapse and neurite retraction in a model system. We further show, using X-ray crystallography, that the ephrin-A5-EphB2 complex is a heterodimer and is architecturally distinct from the tetrameric EphB2-ephrin-B2 structure. The structural data reveal the molecular basis for EphB2-ephrin-A5 signaling and provide a framework for understanding the complexities of functional interactions and crosstalk between A- and B-subclass Eph receptors and ephrins.
About this StructureAbout this Structure
1SHW is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Repelling class discrimination: ephrin-A5 binds to and activates EphB2 receptor signaling., Himanen JP, Chumley MJ, Lackmann M, Li C, Barton WA, Jeffrey PD, Vearing C, Geleick D, Feldheim DA, Boyd AW, Henkemeyer M, Nikolov DB, Nat Neurosci. 2004 May;7(5):501-9. Epub 2004 Apr 25. PMID:15107857
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