1ntb: Difference between revisions
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[[ | ==2.9 A crystal structure of Streptomycin RNA-aptamer complex== | ||
<StructureSection load='1ntb' size='340' side='right' caption='[[1ntb]], [[Resolution|resolution]] 2.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1ntb]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NTB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NTB FirstGlance]. <br> | |||
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SRY:STREPTOMYCIN'>SRY</scene><br> | |||
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1nta|1nta]]</td></tr> | |||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ntb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ntb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ntb RCSB], [http://www.ebi.ac.uk/pdbsum/1ntb PDBsum]</span></td></tr> | |||
<table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We describe a 2.9 A X-ray structure of a complex between the aminocyclitol antibiotic streptomycin and an in vitro selected RNA aptamer, solved using the anomalous diffraction properties of Ba cations. The RNA aptamer, which contains two asymmetric internal loops, adopts a distinct cation-stabilized fold involving a series of S-shaped backbone turns anchored by canonical and noncanonical pairs and triples. The streptomycin streptose ring is encapsulated by stacked arrays of bases from both loops at the elbow of the L-shaped RNA architecture. Specificity is defined by direct hydrogen bonds between all streptose functional groups and base edges that line the inner walls of the cylindrical binding pocket. By contrast, the majority of intermolecular interactions involve contacts to backbone phosphates in the published structure of streptomycin bound to the 16S rRNA. | |||
Encapsulating streptomycin within a small 40-mer RNA.,Tereshko V, Skripkin E, Patel DJ Chem Biol. 2003 Feb;10(2):175-87. PMID:12618190<ref>PMID:12618190</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
< | |||
[[Category: Patel, D J.]] | [[Category: Patel, D J.]] | ||
[[Category: Skripkin, E.]] | [[Category: Skripkin, E.]] | ||
Revision as of 17:02, 28 September 2014
2.9 A crystal structure of Streptomycin RNA-aptamer complex2.9 A crystal structure of Streptomycin RNA-aptamer complex
Structural highlights
Publication Abstract from PubMedWe describe a 2.9 A X-ray structure of a complex between the aminocyclitol antibiotic streptomycin and an in vitro selected RNA aptamer, solved using the anomalous diffraction properties of Ba cations. The RNA aptamer, which contains two asymmetric internal loops, adopts a distinct cation-stabilized fold involving a series of S-shaped backbone turns anchored by canonical and noncanonical pairs and triples. The streptomycin streptose ring is encapsulated by stacked arrays of bases from both loops at the elbow of the L-shaped RNA architecture. Specificity is defined by direct hydrogen bonds between all streptose functional groups and base edges that line the inner walls of the cylindrical binding pocket. By contrast, the majority of intermolecular interactions involve contacts to backbone phosphates in the published structure of streptomycin bound to the 16S rRNA. Encapsulating streptomycin within a small 40-mer RNA.,Tereshko V, Skripkin E, Patel DJ Chem Biol. 2003 Feb;10(2):175-87. PMID:12618190[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
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