1ry4: Difference between revisions

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Revision as of 14:57, 20 March 2008

File:1ry4.jpg

Gene:

Par-6 (Drosophila melanogaster)

Coordinates:

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NMR Structure of the CRIB-PDZ module of Par-6

OverviewOverview

Regulation of protein interaction domains is required for cellular signaling dynamics. Here, we show that the PDZ protein interaction domain from the cell polarity protein Par-6 is regulated by the Rho GTPase Cdc42. Cdc42 binds to a CRIB domain adjacent to the PDZ domain, increasing the affinity of the Par-6 PDZ for its carboxy-terminal ligand by approximately 13-fold. Par-6 PDZ regulation is required for function as mutational disruption of Cdc42-Par-6 PDZ coupling leads to inactivation of Par-6 in polarized MDCK epithelial cells. Structural analysis reveals that the free PDZ domain has several deviations from the canonical PDZ conformation that account for its low ligand affinity. Regulation results from a Cdc42-induced conformational transition in the CRIB-PDZ module that causes the PDZ to assume a canonical, high-affinity PDZ conformation. The coupled CRIB and PDZ architecture of Par-6 reveals how simple binding domains can be combined to yield complex regulation.

About this StructureAbout this Structure

1RY4 is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.

ReferenceReference

Cdc42 regulates the Par-6 PDZ domain through an allosteric CRIB-PDZ transition., Peterson FC, Penkert RR, Volkman BF, Prehoda KE, Mol Cell. 2004 Mar 12;13(5):665-76. PMID:15023337

Page seeded by OCA on Thu Mar 20 13:57:51 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:1ry4.jpg|left|200px]]<br /><applet load="1ry4" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ry4.jpg|left|200px]]
-caption="1ry4" />
-'''NMR Structure of the CRIB-PDZ module of Par-6'''<br />
+ {{Structure
+|PDB= 1ry4 |SIZE=350|CAPTION= <scene name='initialview01'>1ry4</scene>
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE= Par-6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])
+}}
+'''NMR Structure of the CRIB-PDZ module of Par-6'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-RY4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RY4 OCA].
+RY4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RY4 OCA].
 ==Reference==
-Cdc42 regulates the Par-6 PDZ domain through an allosteric CRIB-PDZ transition., Peterson FC, Penkert RR, Volkman BF, Prehoda KE, Mol Cell. 2004 Mar 12;13(5):665-76. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15023337 15023337]
+Cdc42 regulates the Par-6 PDZ domain through an allosteric CRIB-PDZ transition., Peterson FC, Penkert RR, Volkman BF, Prehoda KE, Mol Cell. 2004 Mar 12;13(5):665-76. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15023337 15023337]
 [[Category: Drosophila melanogaster]]
 [[Category: Single protein]]
@@ Line 23: / Line 32: @@
 [[Category: polarity adaptor complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:55:54 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:57:51 2008''