1rl4: Difference between revisions
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[[Image:1rl4.jpg|left|200px]] | [[Image:1rl4.jpg|left|200px]] | ||
'''Plasmodium falciparum peptide deformylase complex with inhibitor''' | {{Structure | ||
|PDB= 1rl4 |SIZE=350|CAPTION= <scene name='initialview01'>1rl4</scene>, resolution 2.18Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=BRR:(2R)-2-{[FORMYL(HYDROXY)AMINO]METHYL}HEXANOIC+ACID'>BRR</scene> and <scene name='pdbligand=BL5:2-{N'-[2-(5-AMINO-1-PHENYLCARBAMOYL-PENTYLCARBAMOYL)-HEXYL]-HYDRAZINOMETHYL}-HEXANOIC ACID(5-AMINO-1-PHENYLCARBAMOYL-PENTYL)-AMIDE'>BL5</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Formylmethionine_deformylase Formylmethionine deformylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.31 3.5.1.31] | |||
|GENE= PDF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36329 Plasmodium falciparum 3D7]) | |||
}} | |||
'''Plasmodium falciparum peptide deformylase complex with inhibitor''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1RL4 is a [ | 1RL4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum_3d7 Plasmodium falciparum 3d7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RL4 OCA]. | ||
==Reference== | ==Reference== | ||
An improved crystal form of Plasmodium falciparum peptide deformylase., Robien MA, Nguyen KT, Kumar A, Hirsh I, Turley S, Pei D, Hol WG, Protein Sci. 2004 Apr;13(4):1155-63. Epub 2004 Mar 9. PMID:[http:// | An improved crystal form of Plasmodium falciparum peptide deformylase., Robien MA, Nguyen KT, Kumar A, Hirsh I, Turley S, Pei D, Hol WG, Protein Sci. 2004 Apr;13(4):1155-63. Epub 2004 Mar 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15010544 15010544] | ||
[[Category: Formylmethionine deformylase]] | [[Category: Formylmethionine deformylase]] | ||
[[Category: Plasmodium falciparum 3d7]] | [[Category: Plasmodium falciparum 3d7]] | ||
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[[Category: plasmodium]] | [[Category: plasmodium]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:53:08 2008'' | ||
Revision as of 14:53, 20 March 2008
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| , resolution 2.18Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Gene: | PDF (Plasmodium falciparum 3D7) | ||||||
| Activity: | Formylmethionine deformylase, with EC number 3.5.1.31 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Plasmodium falciparum peptide deformylase complex with inhibitor
OverviewOverview
An altered version of peptide deformylase from Plasmodium falciparum (PfPDF), the organism that causes the most devastating form of malaria, has been cocrystallized with a synthesized inhibitor that has submicromolar affinity for its target protein. The structure is solved at 2.2 A resolution, an improvement over the 2.8 A resolution achieved during the structural determination of unliganded PfPDF. This represents the successful outcome of modifying the protein construct in order to overcome adverse crystal contacts and other problems encountered in the study of unliganded PfPDF. Two molecules of PfPDF are found in the asymmetric unit of the current structure. The active site of each monomer of PfPDF is occupied by a proteolyzed fragment of the tripeptide-like inhibitor. Unexpectedly, each PfPDF subunit is associated with two nearly complete molecules of the inhibitor, found at a protein-protein interface. This is the first structure of a eukaryotic PDF protein, a potential drug target, in complex with a ligand.
About this StructureAbout this Structure
1RL4 is a Single protein structure of sequence from Plasmodium falciparum 3d7. Full crystallographic information is available from OCA.
ReferenceReference
An improved crystal form of Plasmodium falciparum peptide deformylase., Robien MA, Nguyen KT, Kumar A, Hirsh I, Turley S, Pei D, Hol WG, Protein Sci. 2004 Apr;13(4):1155-63. Epub 2004 Mar 9. PMID:15010544
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