SandboxPKA: Difference between revisions
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Protein kinases are a group of enzymes that possess a catalytic subunit that transfers the gamma (terminal) phosphate from nucleotide triphosphates (often ATP) to one or more amino acid residues in a protein substrate side chain, resulting in a conformational change affecting side protein function. | Protein kinases are a group of enzymes that possess a catalytic subunit that transfers the gamma (terminal) phosphate from nucleotide triphosphates (often ATP) to one or more amino acid residues in a protein substrate side chain, resulting in a conformational change affecting side protein function. | ||
The enzymes are classified into two broad groups, characterised with respect to substrate specificity: | The enzymes are classified into two broad groups, characterised with respect to substrate specificity: | ||
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- '''Serine/threonine kinases''' | - '''Serine/threonine kinases''' | ||
- '''Tyrosine specific kinases''': c-Abl is included in this group | - '''Tyrosine specific kinases''': c-Abl is included in this group | ||
[[Image:reaction.jpg]] | |||
(Leukemia research 34 (10): 1255–1268. doi:10.1016/j.leukres.2010.04.016. PMID 2053738) | (Leukemia research 34 (10): 1255–1268. doi:10.1016/j.leukres.2010.04.016. PMID 2053738) | ||
Revision as of 01:24, 5 December 2012
IntroductionIntroduction
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The c-Abl protein 1 (ABL1), also known as Abelson kinase, is a non-receptor tyrosine kinase that plays a role in many key processes linked to cell growth and survival. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. In more than 90% cases, chronic myelogeneous leukemia (CML) is caused by chromosomal abnormality resulting in the formation of a so-called Philadelphia chromosome. It is caused by fusion between Abelson (Abl) tyrosine kinase gene at chromosome 9 and break point cluster (Bcr) gene at chromosome 22, resulting in the chimeric oncogene Bcr-Abl and a constitutively active Bcr-Abl tyrosine kinase.
ReactionReaction
Protein kinases are a group of enzymes that possess a catalytic subunit that transfers the gamma (terminal) phosphate from nucleotide triphosphates (often ATP) to one or more amino acid residues in a protein substrate side chain, resulting in a conformational change affecting side protein function.
The enzymes are classified into two broad groups, characterised with respect to substrate specificity:
- Serine/threonine kinases - Tyrosine specific kinases: c-Abl is included in this group
(Leukemia research 34 (10): 1255–1268. doi:10.1016/j.leukres.2010.04.016. PMID 2053738)
StructureStructure
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Morado dominio SH3 Rojo dominio SH2
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Catalytic domainCatalytic domain
It is responsible of both, ATP binding as well as protein binding.
Catalitic subunit of c-Abl protein is composed by two different regions: • ATP-binding pocket: is mainly mediated by alfa-helix • Protein-binding pocket: lamina-B domain
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