1r7m: Difference between revisions
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[[Image:1r7m.gif|left|200px]] | [[Image:1r7m.gif|left|200px]] | ||
'''The homing endonuclease I-SceI bound to its DNA recognition region''' | {{Structure | ||
|PDB= 1r7m |SIZE=350|CAPTION= <scene name='initialview01'>1r7m</scene>, resolution 2.25Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | |||
|ACTIVITY= | |||
|GENE= SECY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |||
}} | |||
'''The homing endonuclease I-SceI bound to its DNA recognition region''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1R7M is a [ | 1R7M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R7M OCA]. | ||
==Reference== | ==Reference== | ||
The crystal structure of the gene targeting homing endonuclease I-SceI reveals the origins of its target site specificity., Moure CM, Gimble FS, Quiocho FA, J Mol Biol. 2003 Dec 5;334(4):685-95. PMID:[http:// | The crystal structure of the gene targeting homing endonuclease I-SceI reveals the origins of its target site specificity., Moure CM, Gimble FS, Quiocho FA, J Mol Biol. 2003 Dec 5;334(4):685-95. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14636596 14636596] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:47:55 2008'' | ||
Revision as of 14:47, 20 March 2008
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| , resolution 2.25Å | |||||||
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| Ligands: | |||||||
| Gene: | SECY (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The homing endonuclease I-SceI bound to its DNA recognition region
OverviewOverview
The I-SceI homing endonuclease enhances gene targeting by introducing double-strand breaks at specific chromosomal loci, thereby increasing the recombination frequency. Here, we report the crystal structure of the enzyme complexed to its DNA substrate and Ca(2+) determined at 2.25A resolution. The structure shows the prototypical beta-saddle of LAGLIDADG homing endonucleases that is contributed by two pseudo-symmetric domains. The high specificity of I-SceI is explained by the large number of protein-DNA contacts, many that are made by a long beta-hairpin loop that reaches into the major groove of the DNA. The DNA minor groove is compressed at the catalytic center, bringing the two scissile phosphodiester bonds into close proximity. The protein-Ca(2+)-DNA structure shows the protein bound to its DNA substrate in a pre-reactive state that is defined by the presence of two asymmetric active sites, one of which appears poised to first cleave the DNA bottom strand.
About this StructureAbout this Structure
1R7M is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
The crystal structure of the gene targeting homing endonuclease I-SceI reveals the origins of its target site specificity., Moure CM, Gimble FS, Quiocho FA, J Mol Biol. 2003 Dec 5;334(4):685-95. PMID:14636596
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