4b8t: Difference between revisions
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[[ | ==RNA BINDING PROTEIN Solution structure of the third KH domain of KSRP in complex with the G-rich target sequence.== | ||
<StructureSection load='4b8t' size='340' side='right' caption='[[4b8t]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4b8t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B8T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B8T FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b8t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b8t RCSB], [http://www.ebi.ac.uk/pdbsum/4b8t PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Let-7 is an important tumor-suppressive microRNA (miRNA) that acts as an on-off switch for cellular differentiation and regulates the expression of a set of human oncogenes. Binding of the human KSRP protein to let-7 miRNA precursors positively regulates their processing to mature let-7, thereby contributing to control of cell proliferation, apoptosis and differentiation. Here we analyze the molecular basis for KSRP-let-7 precursor selectivity and show how the third KH domain of the protein recognizes a G-rich sequence in the pre-let-7 terminal loop and dominates the interaction. The structure of the KH3-RNA complex explains the protein recognition of this noncanonical KH target sequence, and we demonstrate that the specificity of this binding is crucial for the functional interaction between the protein and the miRNA precursor. | |||
Noncanonical G recognition mediates KSRP regulation of let-7 biogenesis.,Nicastro G, Garcia-Mayoral MF, Hollingworth D, Kelly G, Martin SR, Briata P, Gherzi R, Ramos A Nat Struct Mol Biol. 2012 Nov 11. doi: 10.1038/nsmb.2427. PMID:23142982<ref>PMID:23142982</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
< | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Briata, P | [[Category: Briata, P]] | ||
[[Category: Garcia-Mayoral, M F | [[Category: Garcia-Mayoral, M F]] | ||
[[Category: Gherzi, R | [[Category: Gherzi, R]] | ||
[[Category: Hollingworth, D | [[Category: Hollingworth, D]] | ||
[[Category: Kelly, G | [[Category: Kelly, G]] | ||
[[Category: Martin, S R | [[Category: Martin, S R]] | ||
[[Category: Nicastro, G | [[Category: Nicastro, G]] | ||
[[Category: Ramos, A | [[Category: Ramos, A]] | ||
[[Category: Transcription-rna complex]] | [[Category: Transcription-rna complex]] | ||
Revision as of 17:40, 9 December 2014
RNA BINDING PROTEIN Solution structure of the third KH domain of KSRP in complex with the G-rich target sequence.RNA BINDING PROTEIN Solution structure of the third KH domain of KSRP in complex with the G-rich target sequence.
Structural highlights
Publication Abstract from PubMedLet-7 is an important tumor-suppressive microRNA (miRNA) that acts as an on-off switch for cellular differentiation and regulates the expression of a set of human oncogenes. Binding of the human KSRP protein to let-7 miRNA precursors positively regulates their processing to mature let-7, thereby contributing to control of cell proliferation, apoptosis and differentiation. Here we analyze the molecular basis for KSRP-let-7 precursor selectivity and show how the third KH domain of the protein recognizes a G-rich sequence in the pre-let-7 terminal loop and dominates the interaction. The structure of the KH3-RNA complex explains the protein recognition of this noncanonical KH target sequence, and we demonstrate that the specificity of this binding is crucial for the functional interaction between the protein and the miRNA precursor. Noncanonical G recognition mediates KSRP regulation of let-7 biogenesis.,Nicastro G, Garcia-Mayoral MF, Hollingworth D, Kelly G, Martin SR, Briata P, Gherzi R, Ramos A Nat Struct Mol Biol. 2012 Nov 11. doi: 10.1038/nsmb.2427. PMID:23142982[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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