Sandbox Reserved 685: Difference between revisions
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results have proved that the mechanism for the formation of argininosuccinate consists of at least two distinct chemical steps with the formation of citrulline adenylate as a reactive intermediate. Argininosuccinate synthetase catalyzes the reversible conversion of citrulline, aspartate, and ATP to argininosuccinate, AMP, and inorganic pyrophosphate. Step 1, activated citrulline-adenylate is formed, releasing inorganic pyrophosphate. Step 2, nucleophilic attack by aspartate amino group forms argininosuccinate and releases AMP.<ref>http://www.jbc.org/content/277/15/13074.full#F1)</ref> | results have proved that the mechanism for the formation of argininosuccinate consists of at least two distinct chemical steps with the formation of citrulline adenylate as a reactive intermediate. Argininosuccinate synthetase catalyzes the reversible conversion of citrulline, aspartate, and ATP to argininosuccinate, AMP, and inorganic pyrophosphate. Step 1, activated citrulline-adenylate is formed, releasing inorganic pyrophosphate. Step 2, nucleophilic attack by aspartate amino group forms argininosuccinate and releases AMP.<ref>http://www.jbc.org/content/277/15/13074.full#F1)</ref> | ||
L-Argininosuccinate, L-histidine, and L-tryptophan inhibited the enzyme activity at saturating amounts of the substrates. L-Norvaline, L-argininosuccinate, L-arginine, L-isoleucine, and L-valine competitively inhibited the enzyme activity at a low concentration of L-citrulline. Argininosuccinate and L-arginine competitively inhibited the enzyme activity at a low concentration of L-aspartate.<ref>http://www.ncbi.nlm.nih.gov/pubmed/447618</ref>This study proved that a number of amino acids can inhibit the Argininosuccinate synthetase enzyme. | |||
===='''Implications'''==== | ===='''Implications'''==== | ||