1q1j: Difference between revisions

Revision as of 14:31, 20 March 2008

, resolution 2.50Å

Coordinates:

Crystal Structure Analysis of anti-HIV-1 Fab 447-52D in complex with V3 peptide

OverviewOverview

447-52D is a human monoclonal antibody isolated from a heterohybridoma derived from an HIV-1-infected individual. This antibody recognizes the hypervariable gp120 V3 loop, and neutralizes both X4 and R5 primary isolates, making it one of the most effective anti-V3 antibodies characterized to date. The crystal structure of the 447-52D Fab in complex with a 16-mer V3 peptide at 2.5 A resolution reveals that the peptide beta hairpin forms a three-stranded mixed beta sheet with complementarity determining region (CDR) H3, with most of the V3 side chains exposed to solvent. Sequence specificity is conferred through interaction of the type-II turn (residues GPGR) at the apex of the V3 hairpin with the base of CDR H3. This novel mode of peptide-antibody recognition enables the antibody to bind to many different V3 sequences where only the GPxR core epitope is absolutely required.

About this StructureAbout this Structure

1Q1J is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structural rationale for the broad neutralization of HIV-1 by human monoclonal antibody 447-52D., Stanfield RL, Gorny MK, Williams C, Zolla-Pazner S, Wilson IA, Structure. 2004 Feb;12(2):193-204. PMID:14962380

Page seeded by OCA on Thu Mar 20 13:31:46 2008

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OCA

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-[[Image:1q1j.gif|left|200px]]<br /><applet load="1q1j" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1q1j.gif|left|200px]]
-caption="1q1j, resolution 2.50&Aring;" />
-'''Crystal Structure Analysis of anti-HIV-1 Fab 447-52D in complex with V3 peptide'''<br />
+ {{Structure
+|PDB= 1q1j |SIZE=350|CAPTION= <scene name='initialview01'>1q1j</scene>, resolution 2.50&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''Crystal Structure Analysis of anti-HIV-1 Fab 447-52D in complex with V3 peptide'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-Q1J is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q1J OCA].
+Q1J is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q1J OCA].
 ==Reference==
-Structural rationale for the broad neutralization of HIV-1 by human monoclonal antibody 447-52D., Stanfield RL, Gorny MK, Williams C, Zolla-Pazner S, Wilson IA, Structure. 2004 Feb;12(2):193-204. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14962380 14962380]
+Structural rationale for the broad neutralization of HIV-1 by human monoclonal antibody 447-52D., Stanfield RL, Gorny MK, Williams C, Zolla-Pazner S, Wilson IA, Structure. 2004 Feb;12(2):193-204. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14962380 14962380]
 [[Category: Homo sapiens]]
 [[Category: Protein complex]]
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 [[Category: v3 loop]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:34:55 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:31:46 2008''