Sandbox Reserved 642: Difference between revisions
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== '''Mechanism''' == | == '''Mechanism''' == | ||
Although the exact mechanism of phenylalanine degradation is still not fully understood, the main reaction requires the addition of an hydroxyl group to the benzene ring of the phenylalanine residue. In order for this process to occur, the cofactor tetrahydrobiopterin(BH4) loses two hydrogen atoms to become dihydrobiopterin. BH4 acts as a reductant by reducing one of the diatomic oxygens while the other is added to the 6-membered ring. In order to stabilize the substrate- enzyme complex as this reaction occurs, an iron atom within the protein is necessary. It is within the active site that the hydrogen atom from phenylalanine is stripped off and replaced with a hydroxyl group. | Although the exact mechanism of phenylalanine degradation is still not fully understood, the main reaction requires the addition of an hydroxyl group to the benzene ring of the phenylalanine residue. In order for this process to occur, the cofactor tetrahydrobiopterin(BH4) loses two hydrogen atoms to become dihydrobiopterin. BH4 acts as a reductant by reducing one of the diatomic oxygens while the other is added to the 6-membered ring. In order to stabilize the substrate- enzyme complex as this reaction occurs, an iron atom within the protein is necessary. It is within the active site that the hydrogen atom from phenylalanine is stripped off and replaced with a hydroxyl group. | ||
[[Image:Phenylalanine_Hydroxylase_mechanism.jpg |thumb| | [[Image:Phenylalanine_Hydroxylase_mechanism.jpg |thumb|450 px|center|Reaction catalyzed by PheOH]] | ||