1pmb: Difference between revisions

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, resolution 2.5Å

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THE DETERMINATION OF THE CRYSTAL STRUCTURE OF RECOMBINANT PIG MYOGLOBIN BY MOLECULAR REPLACEMENT AND ITS REFINEMENT

OverviewOverview

As part of a protein engineering study, the X-ray crystal structure of recombinant pig myoglobin, prepared and crystallized from E. coli, has been determined. Diffraction data were collected to 2.5 A spacing using a synchrotron X-ray source. The structure was solved using the molecular-replacement method and refined using least-squares minimization procedures to a crystallographic R factor of 18.5% using 14,481 reflections between 10 and 2.5 A. A preliminary comparison of the structure of pig myoglobin with other myoglobin structures is presented.

About this StructureAbout this Structure

1PMB is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

ReferenceReference

Determination of the crystal structure of recombinant pig myoglobin by molecular replacement and its refinement., Smerdon SJ, Oldfield TJ, Dodson EJ, Dodson GG, Hubbard RE, Wilkinson AJ, Acta Crystallogr B. 1990 Jun 1;46 ( Pt 3):370-7. PMID:2383370

Page seeded by OCA on Thu Mar 20 13:26:08 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1pmb.gif|left|200px]]<br /><applet load="1pmb" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1pmb.gif|left|200px]]
-caption="1pmb, resolution 2.5&Aring;" />
-'''THE DETERMINATION OF THE CRYSTAL STRUCTURE OF RECOMBINANT PIG MYOGLOBIN BY MOLECULAR REPLACEMENT AND ITS REFINEMENT'''<br />
+ {{Structure
+|PDB= 1pmb |SIZE=350|CAPTION= <scene name='initialview01'>1pmb</scene>, resolution 2.5&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''THE DETERMINATION OF THE CRYSTAL STRUCTURE OF RECOMBINANT PIG MYOGLOBIN BY MOLECULAR REPLACEMENT AND ITS REFINEMENT'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-PMB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PMB OCA].
+PMB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PMB OCA].
 ==Reference==
-Determination of the crystal structure of recombinant pig myoglobin by molecular replacement and its refinement., Smerdon SJ, Oldfield TJ, Dodson EJ, Dodson GG, Hubbard RE, Wilkinson AJ, Acta Crystallogr B. 1990 Jun 1;46 ( Pt 3):370-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2383370 2383370]
+Determination of the crystal structure of recombinant pig myoglobin by molecular replacement and its refinement., Smerdon SJ, Oldfield TJ, Dodson EJ, Dodson GG, Hubbard RE, Wilkinson AJ, Acta Crystallogr B. 1990 Jun 1;46 ( Pt 3):370-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2383370 2383370]
 [[Category: Single protein]]
 [[Category: Sus scrofa]]
@@ Line 22: / Line 31: @@
 [[Category: oxygen storage]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:30:15 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:26:08 2008''