1pl5: Difference between revisions
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[[Image:1pl5.gif|left|200px]] | [[Image:1pl5.gif|left|200px]] | ||
'''Crystal Structure Analysis of the Sir4p C-terminal Coiled Coil''' | {{Structure | ||
|PDB= 1pl5 |SIZE=350|CAPTION= <scene name='initialview01'>1pl5</scene>, resolution 2.5Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= SIR4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |||
}} | |||
'''Crystal Structure Analysis of the Sir4p C-terminal Coiled Coil''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1PL5 is a [ | 1PL5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PL5 OCA]. | ||
==Reference== | ==Reference== | ||
The Sir4 C-terminal coiled coil is required for telomeric and mating type silencing in Saccharomyces cerevisiae., Murphy GA, Spedale EJ, Powell ST, Pillus L, Schultz SC, Chen L, J Mol Biol. 2003 Dec 5;334(4):769-80. PMID:[http:// | The Sir4 C-terminal coiled coil is required for telomeric and mating type silencing in Saccharomyces cerevisiae., Murphy GA, Spedale EJ, Powell ST, Pillus L, Schultz SC, Chen L, J Mol Biol. 2003 Dec 5;334(4):769-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14636601 14636601] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: parallel coiled coil homodimer]] | [[Category: parallel coiled coil homodimer]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:25:48 2008'' | ||
Revision as of 14:25, 20 March 2008
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| , resolution 2.5Å | |||||||
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| Gene: | SIR4 (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure Analysis of the Sir4p C-terminal Coiled Coil
OverviewOverview
Saccharomyces cerevisiae Sir4p plays important roles in silent chromatin at telomeric and silent mating type loci. The C terminus of Sir4p (Sir4CT) is critical for its functions in vivo because over-expression or deletion of Sir4CT fragments disrupts normal telomeric structure and abolishes the telomere position effect. The 2.5A resolution X-ray crystal structure of an Sir4CT fragment (Sir4p 1217-1358) reveals a 72 residue homodimeric, parallel coiled coil, burying an extensive 3600A(2) of surface area. The crystal structure is consistent with results of protein cross-linking and analytical ultracentrifugation results demonstrating that Sir4CT exists as a dimer in solution. Disruption of the coiled coil in vivo by point mutagenesis results in total derepression of telomeric and HML silent mating marker genes, suggesting that coiled coil dimerization is essential for Sir4p-mediated silencing. In addition to the coiled coil dimerization interface (Sir4CC interface), a crystallographic interface between pairs of coiled coils is significantly hydrophobic and buries 1228A(2) of surface area (interface II). Remarkably, interface II mutants are deficient in telomeric silencing but not in mating type silencing in vivo. However, point mutants of interface II do not affect the oligomerization state of Sir4CT in solution. These results are consistent with the hypothesis that interface II mimics a protein interface between Sir4p and one of its protein partners that is essential for telomeric silencing but not mating type silencing.
About this StructureAbout this Structure
1PL5 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
The Sir4 C-terminal coiled coil is required for telomeric and mating type silencing in Saccharomyces cerevisiae., Murphy GA, Spedale EJ, Powell ST, Pillus L, Schultz SC, Chen L, J Mol Biol. 2003 Dec 5;334(4):769-80. PMID:14636601
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