1p5d: Difference between revisions
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'''Enzyme-ligand complex of P. aeruginosa PMM/PGM''' | {{Structure | ||
|PDB= 1p5d |SIZE=350|CAPTION= <scene name='initialview01'>1p5d</scene>, resolution 1.60Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=G1P:ALPHA-D-GLUCOSE-1-PHOSPHATE'>G1P</scene> and <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Phosphomannomutase Phosphomannomutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.8 5.4.2.8] | |||
|GENE= ALGC OR PA5322 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa]) | |||
}} | |||
'''Enzyme-ligand complex of P. aeruginosa PMM/PGM''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1P5D is a [ | 1P5D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P5D OCA]. | ||
==Reference== | ==Reference== | ||
Structural basis of diverse substrate recognition by the enzyme PMM/PGM from P. aeruginosa., Regni C, Naught L, Tipton PA, Beamer LJ, Structure. 2004 Jan;12(1):55-63. PMID:[http:// | Structural basis of diverse substrate recognition by the enzyme PMM/PGM from P. aeruginosa., Regni C, Naught L, Tipton PA, Beamer LJ, Structure. 2004 Jan;12(1):55-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14725765 14725765] | ||
[[Category: Phosphomannomutase]] | [[Category: Phosphomannomutase]] | ||
[[Category: Pseudomonas aeruginosa]] | [[Category: Pseudomonas aeruginosa]] | ||
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[[Category: phosphoserine]] | [[Category: phosphoserine]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:20:06 2008'' | ||
Revision as of 14:20, 20 March 2008
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| , resolution 1.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | ALGC OR PA5322 (Pseudomonas aeruginosa) | ||||||
| Activity: | Phosphomannomutase, with EC number 5.4.2.8 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Enzyme-ligand complex of P. aeruginosa PMM/PGM
OverviewOverview
Enzyme-substrate complexes of phosphomannomutase/phosphoglucomutase (PMM/PGM) reveal the structural basis of the enzyme's ability to use four different substrates in catalysis. High-resolution structures with glucose 1-phosphate, glucose 6-phosphate, mannose 1-phosphate, and mannose 6-phosphate show that the position of the phosphate group of each substrate is held constant by a conserved network of hydrogen bonds. This produces two distinct, and mutually exclusive, binding orientations for the sugar rings of the 1-phospho and 6-phospho sugars. Specific binding of both orientations is accomplished by key contacts with the O3 and O4 hydroxyls of the sugar, which must occupy equatorial positions. Dual recognition of glucose and mannose phosphosugars uses a combination of specific protein contacts and nonspecific solvent contacts. The ability of PMM/PGM to accommodate these four diverse substrates in a single active site is consistent with its highly reversible phosphoryl transfer reaction and allows it to function in multiple biosynthetic pathways in P. aeruginosa.
About this StructureAbout this Structure
1P5D is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis of diverse substrate recognition by the enzyme PMM/PGM from P. aeruginosa., Regni C, Naught L, Tipton PA, Beamer LJ, Structure. 2004 Jan;12(1):55-63. PMID:14725765
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