1oye: Difference between revisions

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Revision as of 14:17, 20 March 2008

File:1oye.gif

, resolution 3.48Å

Ligands:

Gene:

ACRB OR ACRE OR B0462 (Escherichia coli)

Coordinates:

save as pdb, mmCIF, xml

Structural Basis of Multiple Binding Capacity of the AcrB multidrug Efflux Pump

OverviewOverview

Multidrug efflux pumps cause serious problems in cancer chemotherapy and treatment of bacterial infections. Yet high-resolution structures of ligand transporter complexes have previously been unavailable. We obtained x-ray crystallographic structures of the trimeric AcrB pump from Escherichia coli with four structurally diverse ligands. The structures show that three molecules of ligands bind simultaneously to the extremely large central cavity of 5000 cubic angstroms, primarily by hydrophobic, aromatic stacking and van der Waals interactions. Each ligand uses a slightly different subset of AcrB residues for binding. The bound ligand molecules often interact with each other, stabilizing the binding.

About this StructureAbout this Structure

1OYE is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of multiple drug-binding capacity of the AcrB multidrug efflux pump., Yu EW, McDermott G, Zgurskaya HI, Nikaido H, Koshland DE Jr, Science. 2003 May 9;300(5621):976-80. PMID:12738864

Page seeded by OCA on Thu Mar 20 13:17:21 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1oye.gif|left|200px]]<br /><applet load="1oye" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1oye.gif|left|200px]]
-caption="1oye, resolution 3.48&Aring;" />
-'''Structural Basis of Multiple Binding Capacity of the AcrB multidrug Efflux Pump'''<br />
+ {{Structure
+|PDB= 1oye |SIZE=350|CAPTION= <scene name='initialview01'>1oye</scene>, resolution 3.48&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=CPF:1-CYCLOPROPYL-6-FLUORO-4-OXO-7-PIPERAZIN-1-YL-1,4-DIHYDROQUINOLINE-3-CARBOXYLIC ACID'>CPF</scene>
+|ACTIVITY=
+|GENE= ACRB OR ACRE OR B0462 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+}}
+'''Structural Basis of Multiple Binding Capacity of the AcrB multidrug Efflux Pump'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-OYE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CPF:'>CPF</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OYE OCA].
+OYE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OYE OCA].
 ==Reference==
-Structural basis of multiple drug-binding capacity of the AcrB multidrug efflux pump., Yu EW, McDermott G, Zgurskaya HI, Nikaido H, Koshland DE Jr, Science. 2003 May 9;300(5621):976-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12738864 12738864]
+Structural basis of multiple drug-binding capacity of the AcrB multidrug efflux pump., Yu EW, McDermott G, Zgurskaya HI, Nikaido H, Koshland DE Jr, Science. 2003 May 9;300(5621):976-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12738864 12738864]
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
@@ Line 21: / Line 30: @@
 [[Category: membrane protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:23:01 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:17:21 2008''