1oa5: Difference between revisions
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[[Image:1oa5.jpg|left|200px]] | [[Image:1oa5.jpg|left|200px]] | ||
'''THE SOLUTION STRUCTURE OF BOVINE PANCREATIC TRYPSIN INHIBITOR AT HIGH PRESSURE''' | {{Structure | ||
|PDB= 1oa5 |SIZE=350|CAPTION= <scene name='initialview01'>1oa5</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= | |||
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'''THE SOLUTION STRUCTURE OF BOVINE PANCREATIC TRYPSIN INHIBITOR AT HIGH PRESSURE''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1OA5 is a [ | 1OA5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OA5 OCA]. | ||
==Reference== | ==Reference== | ||
The solution structure of bovine pancreatic trypsin inhibitor at high pressure., Williamson MP, Akasaka K, Refaee M, Protein Sci. 2003 Sep;12(9):1971-9. PMID:[http:// | The solution structure of bovine pancreatic trypsin inhibitor at high pressure., Williamson MP, Akasaka K, Refaee M, Protein Sci. 2003 Sep;12(9):1971-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12930996 12930996] | ||
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protease inhibitor]] | [[Category: protease inhibitor]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:07:57 2008'' | ||
Revision as of 14:07, 20 March 2008
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THE SOLUTION STRUCTURE OF BOVINE PANCREATIC TRYPSIN INHIBITOR AT HIGH PRESSURE
OverviewOverview
The solution structure of bovine pancreatic trypsin inhibitor (BPTI) at a pressure of 2 kbar is presented. The structure was calculated as a change from an energy-minimized low-pressure structure, using (1)H chemical shifts as restraints. The structure has changed by 0.24 A RMS, and has almost unchanged volume. The largest changes as a result of pressure are in the loop 10-16, which contains the active site of BPTI, and residues 38-42, which are adjacent to buried water molecules. Hydrogen bonds are compressed by 0.029 +/- 0.117 A, with the longer hydrogen bonds, including those to internal buried water molecules, being compressed more. The hydrophobic core is also compressed, largely from reduction of packing defects. The parts of the structure that have the greatest change are close to buried water molecules, thus highlighting the importance of water molecules as the nucleation sites for volume fluctuation of proteins in native conditions.
About this StructureAbout this Structure
1OA5 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
ReferenceReference
The solution structure of bovine pancreatic trypsin inhibitor at high pressure., Williamson MP, Akasaka K, Refaee M, Protein Sci. 2003 Sep;12(9):1971-9. PMID:12930996
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