1o7z: Difference between revisions

Revision as of 14:07, 20 March 2008

, resolution 1.92Å

Coordinates:

CRYSTAL STRUCTURE OF IP-10 T-FORM

OverviewOverview

We have determined the structure of wild-type IP-10 from three crystal forms. The crystals provide eight separate models of the IP-10 chain, all differing substantially from a monomeric IP-10 variant examined previously by NMR spectroscopy. In each crystal form, IP-10 chains form conventional beta sheet dimers, which, in turn, form a distinct tetrameric assembly. The M form tetramer is reminiscent of platelet factor 4, whereas the T and H forms feature a novel twelve-stranded beta sheet. Analytical ultracentrifugation indicates that, in free solution, IP-10 exists in a monomer-dimer equilibrium with a dissociation constant of 9 microM. We propose that the tetrameric structures may represent species promoted by the binding of glycosaminoglycans. The binding sites for several IP-10-neutralizing mAbs have also been mapped.

About this StructureAbout this Structure

1O7Z is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of oligomeric forms of the IP-10/CXCL10 chemokine., Swaminathan GJ, Holloway DE, Colvin RA, Campanella GK, Papageorgiou AC, Luster AD, Acharya KR, Structure. 2003 May;11(5):521-32. PMID:12737818

Page seeded by OCA on Thu Mar 20 13:07:00 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1o7z.jpg|left|200px]]<br /><applet load="1o7z" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1o7z.jpg|left|200px]]
-caption="1o7z, resolution 1.92&Aring;" />
-'''CRYSTAL STRUCTURE OF IP-10 T-FORM'''<br />
+ {{Structure
+|PDB= 1o7z |SIZE=350|CAPTION= <scene name='initialview01'>1o7z</scene>, resolution 1.92&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''CRYSTAL STRUCTURE OF IP-10 T-FORM'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-O7Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7Z OCA].
+O7Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7Z OCA].
 ==Reference==
-Crystal structures of oligomeric forms of the IP-10/CXCL10 chemokine., Swaminathan GJ, Holloway DE, Colvin RA, Campanella GK, Papageorgiou AC, Luster AD, Acharya KR, Structure. 2003 May;11(5):521-32. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12737818 12737818]
+Crystal structures of oligomeric forms of the IP-10/CXCL10 chemokine., Swaminathan GJ, Holloway DE, Colvin RA, Campanella GK, Papageorgiou AC, Luster AD, Acharya KR, Structure. 2003 May;11(5):521-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12737818 12737818]
 [[Category: Single protein]]
 [[Category: Acharya, K R.]]
@@ Line 21: / Line 30: @@
 [[Category: interferon induction]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:14:31 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:07:00 2008''