1hcj: Difference between revisions

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-[[Image:1hcj.png|left|200px]]
+==Photoproduct of the wild-type Aequorea victoria Green Fluorescent Protein==
+<StructureSection load='1hcj' size='340' side='right' caption='[[1hcj]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1hcj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HCJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HCJ FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ABA:ALPHA-AMINOBUTYRIC+ACID'>ABA</scene>, <scene name='pdbligand=GYS:[(4Z)-2-(1-AMINO-2-HYDROXYETHYL)-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYS</scene></td></tr>
+<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1b9c|1b9c]], [[1bfp|1bfp]], [[1c4f|1c4f]], [[1emb|1emb]], [[1emc|1emc]], [[1eme|1eme]], [[1emf|1emf]], [[1emg|1emg]], [[1emk|1emk]], [[1eml|1eml]], [[1emm|1emm]], [[1f09|1f09]], [[1f0b|1f0b]], [[1yfp|1yfp]], [[2emd|2emd]], [[2emn|2emn]], [[2emo|2emo]]</td></tr>
+<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hcj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hcj RCSB], [http://www.ebi.ac.uk/pdbsum/1hcj PDBsum]</span></td></tr>
+<table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hc/1hcj_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Wild type green fluorescent protein (GFP) from Aequorea victoria absorbs predominantly at 398 nm. Illumination with UV (254 nm) or visible (390 nm) light transforms this state (GFP(398)) into one absorbing at 483 nm (GFP(483)). Here we show that this photoconversion of GFP is a one-photon process that is paralleled by decarboxylation of Glu 222. We propose a mechanism in which decarboxylation is due to electron transfer between the gamma-carboxylate of Glu 222 and the p-hydroxybenzylidene-imidazolidinone chromophore of GFP, followed by reverse transfer of an electron and a proton to the remaining carbon side chain atom of Glu 222. Oxidative decarboxylation of a gamma-carboxylate represents a new type of posttranslational modification that may also occur in enzymes with high-potential reaction intermediates.
-{{STRUCTURE_1hcj|  PDB=1hcj  |  SCENE=  }}
+Phototransformation of green fluorescent protein with UV and visible light leads to decarboxylation of glutamate 222.,van Thor JJ, Gensch T, Hellingwerf KJ, Johnson LN Nat Struct Biol. 2002 Jan;9(1):37-41. PMID:11740505<ref>PMID:11740505</ref>
-===PHOTOPRODUCT OF THE WILD-TYPE AEQUOREA VICTORIA GREEN FLUORESCENT PROTEIN===
+From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_11740505}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[1hcj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HCJ OCA].
+</StructureSection>
+[[Category: Aeqvi]]
-==See Also==
-*[[Alyssa Marsico/Sandbox 1|Alyssa Marsico/Sandbox 1]]
-*[[Devon McCarthy/Sandbox 1|Devon McCarthy/Sandbox 1]]
-*[[Green Fluorescent Protein|Green Fluorescent Protein]]
-*[[Sandbox104|Sandbox104]]
-*[[User:Joanne Lau/Sandbox 5|User:Joanne Lau/Sandbox 5]]
-==Reference==
-<ref group="xtra">PMID:011740505</ref><references group="xtra"/>
-[[Category: Aequorea victoria]]
 [[Category: Gensch, T.]]
 [[Category: Hellingwerf, K J.]]