1ny4: Difference between revisions

Revision as of 14:03, 20 March 2008

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Solution structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii. Northeast Structural Genomics Consortium target JR19.

OverviewOverview

We report NMR assignments and solution structure of the 71-residue 30S ribosomal protein S28E from the archaean Pyrococcus horikoshii, target JR19 of the Northeast Structural Genomics Consortium. The structure, determined rapidly with the aid of automated backbone resonance assignment (AutoAssign) and automated structure determination (AutoStructure) software, is characterized by a four-stranded beta-sheet with a classic Greek-key topology and an oligonucleotide/oligosaccharide beta-barrel (OB) fold. The electrostatic surface of S28E exhibits positive and negative patches on opposite sides, the former constituting a putative binding site for RNA. The 13 C-terminal residues of the protein contain a consensus sequence motif constituting the signature of the S28E protein family. Surprisingly, this C-terminal segment is unstructured in solution.

About this StructureAbout this Structure

1NY4 is a Single protein structure of sequence from Pyrococcus horikoshii. Full crystallographic information is available from OCA.

ReferenceReference

Solution NMR structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii., Aramini JM, Huang YJ, Cort JR, Goldsmith-Fischman S, Xiao R, Shih LY, Ho CK, Liu J, Rost B, Honig B, Kennedy MA, Acton TB, Montelione GT, Protein Sci. 2003 Dec;12(12):2823-30. PMID:14627742

Page seeded by OCA on Thu Mar 20 13:03:11 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1ny4.gif|left|200px]]<br /><applet load="1ny4" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ny4.gif|left|200px]]
-caption="1ny4" />
-'''Solution structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii. Northeast Structural Genomics Consortium target JR19.'''<br />
+ {{Structure
+|PDB= 1ny4 |SIZE=350|CAPTION= <scene name='initialview01'>1ny4</scene>
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE= RPS28E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii])
+}}
+'''Solution structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii. Northeast Structural Genomics Consortium target JR19.'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-NY4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NY4 OCA].
+NY4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NY4 OCA].
 ==Reference==
-Solution NMR structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii., Aramini JM, Huang YJ, Cort JR, Goldsmith-Fischman S, Xiao R, Shih LY, Ho CK, Liu J, Rost B, Honig B, Kennedy MA, Acton TB, Montelione GT, Protein Sci. 2003 Dec;12(12):2823-30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14627742 14627742]
+Solution NMR structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii., Aramini JM, Huang YJ, Cort JR, Goldsmith-Fischman S, Xiao R, Shih LY, Ho CK, Liu J, Rost B, Honig B, Kennedy MA, Acton TB, Montelione GT, Protein Sci. 2003 Dec;12(12):2823-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14627742 14627742]
 [[Category: Pyrococcus horikoshii]]
 [[Category: Single protein]]
@@ Line 32: / Line 41: @@
 [[Category: ribosomal protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:11:18 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:03:11 2008''