1nt2: Difference between revisions

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Revision as of 14:01, 20 March 2008

File:1nt2.jpg

, resolution 2.90Å

Ligands:

Gene:

AF2087 (Archaeoglobus fulgidus), AF2088 (Archaeoglobus fulgidus)

Coordinates:

save as pdb, mmCIF, xml

CRYSTAL STRUCTURE OF FIBRILLARIN/NOP5P COMPLEX

OverviewOverview

Nop56p and Nop58p are two core proteins of the box C/D snoRNPs that interact concurrently with fibrillarin and snoRNAs to function in enzyme assembly and catalysis. Here we report the 2.9 A resolution co-crystal structure of an archaeal homolog of Nop56p/Nop58p, Nop5p, in complex with fibrillarin from Archaeoglobus fulgidus (AF) and the methyl donor S-adenosyl-L-methionine. The N-terminal domain of Nop5p forms a complementary surface to fibrillarin that serves to anchor the catalytic subunit and to stabilize cofactor binding. A coiled coil in Nop5p mediates dimerization of two fibrillarin-Nop5p heterodimers for optimal interactions with bipartite box C/D RNAs. Structural analysis and complementary biochemical data demonstrate that the conserved C-terminal domain of Nop5p harbors RNA-binding sites. A model of box C/D snoRNP assembly is proposed based on the presented structural and biochemical data.

About this StructureAbout this Structure

1NT2 is a Protein complex structure of sequences from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.

ReferenceReference

Structure and function of archaeal box C/D sRNP core proteins., Aittaleb M, Rashid R, Chen Q, Palmer JR, Daniels CJ, Li H, Nat Struct Biol. 2003 Apr;10(4):256-63. PMID:12598892

Page seeded by OCA on Thu Mar 20 13:01:18 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1nt2.jpg|left|200px]]<br /><applet load="1nt2" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1nt2.jpg|left|200px]]
-caption="1nt2, resolution 2.90&Aring;" />
-'''CRYSTAL STRUCTURE OF FIBRILLARIN/NOP5P COMPLEX'''<br />
+ {{Structure
+|PDB= 1nt2 |SIZE=350|CAPTION= <scene name='initialview01'>1nt2</scene>, resolution 2.90&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>
+|ACTIVITY=
+|GENE= AF2087 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2234 Archaeoglobus fulgidus]), AF2088 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2234 Archaeoglobus fulgidus])
+}}
+'''CRYSTAL STRUCTURE OF FIBRILLARIN/NOP5P COMPLEX'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-NT2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus] with <scene name='pdbligand=SAM:'>SAM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NT2 OCA].
+NT2 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NT2 OCA].
 ==Reference==
-Structure and function of archaeal box C/D sRNP core proteins., Aittaleb M, Rashid R, Chen Q, Palmer JR, Daniels CJ, Li H, Nat Struct Biol. 2003 Apr;10(4):256-63. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12598892 12598892]
+Structure and function of archaeal box C/D sRNP core proteins., Aittaleb M, Rashid R, Chen Q, Palmer JR, Daniels CJ, Li H, Nat Struct Biol. 2003 Apr;10(4):256-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12598892 12598892]
 [[Category: Archaeoglobus fulgidus]]
 [[Category: Protein complex]]
@@ Line 24: / Line 33: @@
 [[Category: coiled coil]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:09:42 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:01:18 2008''