5bj3: Difference between revisions

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-[[Image:5bj3.png|left|200px]]
+==THERMUS THERMOPHILUS ASPARTATE AMINOTRANSFERASE TETRA MUTANT 1==
+<StructureSection load='5bj3' size='340' side='right' caption='[[5bj3]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5bj3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BJ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BJ3 FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bj3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5bj3 RCSB], [http://www.ebi.ac.uk/pdbsum/5bj3 PDBsum]</span></td></tr>
+</table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bj/5bj3_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Aspartate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus HB8 (ttAspAT), has been believed to be specific for an acidic substrate. However, stepwise introduction of mutations in the active-site residues finally changed its substrate specificity to that of a dual-substrate enzyme. The final mutant, [S15D, T17V, K109S, S292R] ttAspAT, is active toward both acidic and hydrophobic substrates. During the course of stepwise mutation, the activities toward acidic and hydrophobic substrates changed independently. The introduction of a mobile Arg292* residue into ttAspAT was the key step in the change to a "dual-substrate" enzyme. The substrate recognition mechanism of this thermostable "dual-substrate" enzyme was confirmed by X-ray crystallography. This work together with previous studies on various enzymes suggest that this unique "dual-substrate recognition" mechanism is a feature of not only aminotransferases but also other enzymes.
-{{STRUCTURE_5bj3|  PDB=5bj3  |  SCENE=  }}
+Substrate recognition mechanism of thermophilic dual-substrate enzyme.,Ura H, Nakai T, Kawaguchi SI, Miyahara I, Hirotsu K, Kuramitsu S J Biochem. 2001 Jul;130(1):89-98. PMID:11432784<ref>PMID:11432784</ref>
-===THERMUS THERMOPHILUS ASPARTATE AMINOTRANSFERASE TETRA MUTANT 1===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_11432784}}
-==About this Structure==
-[[5bj3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BJ3 OCA].
 ==See Also==
 *[[Aspartate Aminotransferase|Aspartate Aminotransferase]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:011432784</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Aspartate transaminase]]
 [[Category: Thermus aquaticus]]
-[[Category: Hirotsu, K.]]
+[[Category: Hirotsu, K]]
-[[Category: Kawaguchi, S I.]]
+[[Category: Kawaguchi, S I]]
-[[Category: Kuramitsu, S.]]
+[[Category: Kuramitsu, S]]
-[[Category: Miyahara, I.]]
+[[Category: Miyahara, I]]
-[[Category: Nakai, T.]]
+[[Category: Nakai, T]]
-[[Category: Ura, H.]]
+[[Category: Ura, H]]
 [[Category: Aminotransferase]]
 [[Category: Pyridoxal enzyme]]
 [[Category: Transferase]]