1nsf: Difference between revisions
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'''D2 HEXAMERIZATION DOMAIN OF N-ETHYLMALEIMIDE SENSITIVE FACTOR (NSF)''' | {{Structure | ||
|PDB= 1nsf |SIZE=350|CAPTION= <scene name='initialview01'>1nsf</scene>, resolution 1.90Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=ATP:ADENOSINE-5'-TRIPHOSPHATE'>ATP</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''D2 HEXAMERIZATION DOMAIN OF N-ETHYLMALEIMIDE SENSITIVE FACTOR (NSF)''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1NSF is a [ | 1NSF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. The following page contains interesting information on the relation of 1NSF with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb80_1.html AAA+ Proteases]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NSF OCA]. | ||
==Reference== | ==Reference== | ||
Structure of the ATP-dependent oligomerization domain of N-ethylmaleimide sensitive factor complexed with ATP., Yu RC, Hanson PI, Jahn R, Brunger AT, Nat Struct Biol. 1998 Sep;5(9):803-11. PMID:[http:// | Structure of the ATP-dependent oligomerization domain of N-ethylmaleimide sensitive factor complexed with ATP., Yu RC, Hanson PI, Jahn R, Brunger AT, Nat Struct Biol. 1998 Sep;5(9):803-11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9731775 9731775] | ||
[[Category: AAA+ Proteases]] | [[Category: AAA+ Proteases]] | ||
[[Category: Cricetulus griseus]] | [[Category: Cricetulus griseus]] | ||
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[[Category: protein transport]] | [[Category: protein transport]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:01:02 2008'' | ||
Revision as of 14:01, 20 March 2008
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D2 HEXAMERIZATION DOMAIN OF N-ETHYLMALEIMIDE SENSITIVE FACTOR (NSF)
OverviewOverview
N-ethylmaleimide-sensitive factor (NSF) is a hexameric ATPase which primes and/or dissociates SNARE complexes involved in intracellular fusion events. Each NSF protomer contains three domains: an N-terminal domain required for SNARE binding and two ATPase domains, termed D1 and D2, with D2 being required for oligomerization. We have determined the 1.9 A crystal structure of the D2 domain of NSF complexed with ATP using multi-wavelength anomalous dispersion phasing. D2 consists of a nucleotide binding subdomain with a Rossmann fold and a C-terminal subdomain, which is structurally unique among nucleotide binding proteins. There are interactions between the ATP moiety and both the neighboring D2 protomer and the C-terminal subdomain that may be important for ATP-dependent oligomerization. Of particular importance are three well-ordered and conserved lysine residues that form ionic interactions with the beta- and gamma-phosphates, one of which likely contributes to the low hydrolytic activity of D2.
About this StructureAbout this Structure
1NSF is a Single protein structure of sequence from Cricetulus griseus. The following page contains interesting information on the relation of 1NSF with [AAA+ Proteases]. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the ATP-dependent oligomerization domain of N-ethylmaleimide sensitive factor complexed with ATP., Yu RC, Hanson PI, Jahn R, Brunger AT, Nat Struct Biol. 1998 Sep;5(9):803-11. PMID:9731775
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