1no4: Difference between revisions
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[[Image:1no4.gif|left|200px]] | [[Image:1no4.gif|left|200px]] | ||
'''Crystal Structure of the pre-assembly scaffolding protein gp7 from the double-stranded DNA bacteriophage phi29''' | {{Structure | ||
|PDB= 1no4 |SIZE=350|CAPTION= <scene name='initialview01'>1no4</scene>, resolution 2.20Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= gp7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=221993 Vibrio phage f237]) | |||
}} | |||
'''Crystal Structure of the pre-assembly scaffolding protein gp7 from the double-stranded DNA bacteriophage phi29''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1NO4 is a [ | 1NO4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_phage_f237 Vibrio phage f237]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NO4 OCA]. | ||
==Reference== | ==Reference== | ||
Bacteriophage phi29 scaffolding protein gp7 before and after prohead assembly., Morais MC, Kanamaru S, Badasso MO, Koti JS, Owen BA, McMurray CT, Anderson DL, Rossmann MG, Nat Struct Biol. 2003 Jul;10(7):572-6. PMID:[http:// | Bacteriophage phi29 scaffolding protein gp7 before and after prohead assembly., Morais MC, Kanamaru S, Badasso MO, Koti JS, Owen BA, McMurray CT, Anderson DL, Rossmann MG, Nat Struct Biol. 2003 Jul;10(7):572-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12778115 12778115] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Vibrio phage f237]] | [[Category: Vibrio phage f237]] | ||
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[[Category: coiled-coil]] | [[Category: coiled-coil]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:59:23 2008'' | ||
Revision as of 13:59, 20 March 2008
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| , resolution 2.20Å | |||||||
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| Gene: | gp7 (Vibrio phage f237) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the pre-assembly scaffolding protein gp7 from the double-stranded DNA bacteriophage phi29
OverviewOverview
Three-dimensional structures of the double-stranded DNA bacteriophage phi29 scaffolding protein (gp7) before and after prohead assembly have been determined at resolutions of 2.2 and 2.8 A, respectively. Both structures are dimers that resemble arrows, with a four-helix bundle composing the arrowhead and a coiled coil forming the tail. The structural resemblance of gp7 to the yeast transcription factor GCN4 suggests a DNA-binding function that was confirmed by native gel electrophoresis. DNA binding to gp7 may have a role in mediating the structural transition from prohead to mature virus and scaffold release. A cryo-EM analysis indicates that gp7 is arranged inside the capsid as a series of concentric shells. The position of the higher density features in these shells correlates with the positions of hexamers in the equatorial region of the capsid, suggesting that gp7 may regulate formation of the prolate head through interactions with these hexamers.
About this StructureAbout this Structure
1NO4 is a Single protein structure of sequence from Vibrio phage f237. Full crystallographic information is available from OCA.
ReferenceReference
Bacteriophage phi29 scaffolding protein gp7 before and after prohead assembly., Morais MC, Kanamaru S, Badasso MO, Koti JS, Owen BA, McMurray CT, Anderson DL, Rossmann MG, Nat Struct Biol. 2003 Jul;10(7):572-6. PMID:12778115
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