1njr: Difference between revisions

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Revision as of 13:57, 20 March 2008

File:1njr.gif

, resolution 1.9Å

Ligands:

Gene:

YMR087W or YM9582.12 (Saccharomyces cerevisiae)

Coordinates:

save as pdb, mmCIF, xml

Crystal structure of yeast ymx7, an ADP-ribose-1-monophosphatase

OverviewOverview

Appr-1-pase, an important and ubiquitous cellular processing enzyme involved in the tRNA splicing pathway, catalyzes the conversion of ADP-ribose-1monophosphate (Appr-1-p) to ADP-ribose. The structures of the native enzyme from the yeast and its complex with ADP-ribose were determined to 1.9 A and 2.05 A, respectively. Analysis of the three-dimensional structure of this protein, selected as a target in a structural genomics project, reveals its putative function and provides clues to the catalytic mechanism. The structure of the 284-amino acid protein shows a two-domain architecture consisting of a three-layer alphabetaalpha sandwich N-terminal domain connected to a small C-terminal helical domain. The structure of Appr-1-pase in complex with the product, ADP-ribose, reveals an active-site water molecule poised for nucleophilic attack on the terminal phosphate group. Loop-region residues Asn 80, Asp 90, and His 145 may form a catalytic triad.

About this StructureAbout this Structure

1NJR is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

Structure and mechanism of ADP-ribose-1-monophosphatase (Appr-1-pase), a ubiquitous cellular processing enzyme., Kumaran D, Eswaramoorthy S, Studier FW, Swaminathan S, Protein Sci. 2005 Mar;14(3):719-26. PMID:15722447

Page seeded by OCA on Thu Mar 20 12:57:48 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1njr.gif|left|200px]]<br /><applet load="1njr" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1njr.gif|left|200px]]
-caption="1njr, resolution 1.9&Aring;" />
-'''Crystal structure of yeast ymx7, an ADP-ribose-1''-monophosphatase'''<br />
+ {{Structure
+|PDB= 1njr |SIZE=350|CAPTION= <scene name='initialview01'>1njr</scene>, resolution 1.9&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=XYL:D-XYLITOL'>XYL</scene>
+|ACTIVITY=
+|GENE= YMR087W or YM9582.12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
+}}
+'''Crystal structure of yeast ymx7, an ADP-ribose-1''-monophosphatase'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-NJR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=XYL:'>XYL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NJR OCA].
+NJR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NJR OCA].
 ==Reference==
-Structure and mechanism of ADP-ribose-1''-monophosphatase (Appr-1''-pase), a ubiquitous cellular processing enzyme., Kumaran D, Eswaramoorthy S, Studier FW, Swaminathan S, Protein Sci. 2005 Mar;14(3):719-26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15722447 15722447]
+Structure and mechanism of ADP-ribose-1''-monophosphatase (Appr-1''-pase), a ubiquitous cellular processing enzyme., Kumaran D, Eswaramoorthy S, Studier FW, Swaminathan S, Protein Sci. 2005 Mar;14(3):719-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15722447 15722447]
 [[Category: Saccharomyces cerevisiae]]
 [[Category: Single protein]]
@@ Line 25: / Line 34: @@
 [[Category: protein structure initiative]]
 [[Category: psi]]
-[[Category: structural genomics]]
+[[Category: structural genomic]]
 [[Category: two domain organization]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:06:49 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:57:48 2008''