1nhc: Difference between revisions
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[[Image:1nhc.gif|left|200px]] | [[Image:1nhc.gif|left|200px]] | ||
'''Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger''' | {{Structure | ||
|PDB= 1nhc |SIZE=350|CAPTION= <scene name='initialview01'>1nhc</scene>, resolution 1.70Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Polygalacturonase Polygalacturonase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.15 3.2.1.15] | |||
|GENE= | |||
}} | |||
'''Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1NHC is a [ | 1NHC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NHC OCA]. | ||
==Reference== | ==Reference== | ||
Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger., van Pouderoyen G, Snijder HJ, Benen JA, Dijkstra BW, FEBS Lett. 2003 Nov 20;554(3):462-6. PMID:[http:// | Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger., van Pouderoyen G, Snijder HJ, Benen JA, Dijkstra BW, FEBS Lett. 2003 Nov 20;554(3):462-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14623112 14623112] | ||
[[Category: Aspergillus niger]] | [[Category: Aspergillus niger]] | ||
[[Category: Polygalacturonase]] | [[Category: Polygalacturonase]] | ||
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[[Category: beta-helix]] | [[Category: beta-helix]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:56:54 2008'' | ||
Revision as of 13:56, 20 March 2008
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| , resolution 1.70Å | |||||||
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| Ligands: | , , and | ||||||
| Activity: | Polygalacturonase, with EC number 3.2.1.15 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger
OverviewOverview
Endopolygalacturonase I is a processive enzyme, while the 60% sequence identical endopolygalacturonase II is not. The 1.70 A resolution crystal structure of endopolygalacturonase I reveals a narrowed substrate binding cleft. In addition, Arg96, a residue in this cleft previously shown to be critical for processivity, interacts with the substrate mimics glycerol and sulfate in several well-defined conformations in the six molecules in the asymmetric unit. From this we conclude that both Arg96 and the narrowed substrate binding cleft contribute to retaining the substrate while it moves through the active site after a cleavage event has occurred.
About this StructureAbout this Structure
1NHC is a Single protein structure of sequence from Aspergillus niger. Full crystallographic information is available from OCA.
ReferenceReference
Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger., van Pouderoyen G, Snijder HJ, Benen JA, Dijkstra BW, FEBS Lett. 2003 Nov 20;554(3):462-6. PMID:14623112
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