1ngy: Difference between revisions

Revision as of 13:56, 20 March 2008

, resolution 2.20Å

Coordinates:

Chimeric Mature Fab 7g12-Apo

OverviewOverview

The crystal structure of the Michaelis complex between the Fab fragment of ferrochelatase antibody 7G12 and its substrate mesoporphyrin has been solved to 2.6-A resolution. The antibody-bound mesoporphyrin clearly adopts a nonplanar conformation and reveals that the antibody catalyzes the porphyrin metallation reaction by straining/distorting the bound substrate toward the transition-state configuration. The crystal structures of the Fab fragment of the germ-line precursor antibody to 7G12 and its complex with the hapten N-methylmesoporphyrin have also been solved. A comparison of these structures with the corresponding structures of the affinity-matured antibody 7G12 reveals the molecular mechanism by which the immune system evolves binding energy to catalyze this reaction.

About this StructureAbout this Structure

1NGY is a Protein complex structure of sequences from Mus musculus, homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structural evidence for substrate strain in antibody catalysis., Yin J, Andryski SE, Beuscher AE 4th, Stevens RC, Schultz PG, Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):856-61. Epub 2003 Jan 24. PMID:12552112

Page seeded by OCA on Thu Mar 20 12:56:48 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1ngy.gif|left|200px]]<br /><applet load="1ngy" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ngy.gif|left|200px]]
-caption="1ngy, resolution 2.20&Aring;" />
-'''Chimeric Mature Fab 7g12-Apo'''<br />
+ {{Structure
+|PDB= 1ngy |SIZE=350|CAPTION= <scene name='initialview01'>1ngy</scene>, resolution 2.20&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''Chimeric Mature Fab 7g12-Apo'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-NGY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus,_homo_sapiens Mus musculus, homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGY OCA].
+NGY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus,_homo_sapiens Mus musculus, homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGY OCA].
 ==Reference==
-Structural evidence for substrate strain in antibody catalysis., Yin J, Andryski SE, Beuscher AE 4th, Stevens RC, Schultz PG, Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):856-61. Epub 2003 Jan 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12552112 12552112]
+Structural evidence for substrate strain in antibody catalysis., Yin J, Andryski SE, Beuscher AE 4th, Stevens RC, Schultz PG, Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):856-61. Epub 2003 Jan 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12552112 12552112]
 [[Category: Mus musculus, homo sapiens]]
 [[Category: Protein complex]]
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 [[Category: immunoglobulin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:05:58 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:56:48 2008''