3plh: Difference between revisions
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[[ | ==urate oxidase under 1.5 MPa / 15 bars pressure of equimolar mixture xenon : nitrous oxide== | ||
<StructureSection load='3plh' size='340' side='right' caption='[[3plh]], [[Resolution|resolution]] 1.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3plh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_flavus Aspergillus flavus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PLH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PLH FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AZA:8-AZAXANTHINE'>AZA</scene>, <scene name='pdbligand=N2O:NITROUS+OXIDE'>N2O</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=XE:XENON'>XE</scene></td></tr> | |||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3pjk|3pjk]], [[3pk3|3pk3]], [[3pk4|3pk4]], [[3pk5|3pk5]], [[3pk6|3pk6]], [[3pk8|3pk8]], [[3pkf|3pkf]], [[3pkg|3pkg]], [[3pkh|3pkh]], [[3pkk|3pkk]], [[3pkl|3pkl]], [[3pks|3pks]], [[3pkt|3pkt]], [[3pku|3pku]], [[3ple|3ple]], [[3plg|3plg]], [[3pli|3pli]], [[3plj|3plj]], [[3plm|3plm]], [[2ic0|2ic0]], [[2icq|2icq]], [[2iba|2iba]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">uaZ, uox ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5059 Aspergillus flavus])</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Urate_oxidase Urate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.3 1.7.3.3] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3plh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3plh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3plh RCSB], [http://www.ebi.ac.uk/pdbsum/3plh PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The remarkably safe anesthetics xenon (Xe) and, to lesser extent, nitrous oxide (N(2)O) possess neuroprotective properties in preclinical studies. To investigate the mechanisms of pharmacological action of these gases, which are still poorly known, we performed both crystallography under a large range of gas pressure and biochemical studies on urate oxidase, a prototype of globular gas-binding proteins whose activity is modulated by inert gases. We show that Xe and N(2)O bind to, compete for, and expand the volume of a hydrophobic cavity located just behind the active site of urate oxidase and further inhibit urate oxidase enzymatic activity. By demonstrating a significant relationship between the binding and biochemical effects of Xe and N(2)O, given alone or in combination, these data from structure to function highlight the mechanisms by which chemically and metabolically inert gases can alter protein function and produce their pharmacological effects. Interestingly, the effects of a Xe:N(2)O equimolar mixture were found to be equivalent to those of Xe alone, thereby suggesting that gas mixtures containing Xe and N(2)O could be an alternative and efficient neuroprotective strategy to Xe alone, whose widespread clinical use is limited due to the cost of production and availability of this gas.-Marassio, G., Prange, T., David, H. N., Sopkova-de Oliveira Santos, J., Gabison, L., Delcroix, N., Abraini, J. H., Colloc'h, N. Pressure-response analysis of anesthetic gases xenon and nitrous oxide on urate oxidase: a crystallographic study. | |||
Pressure-response analysis of anesthetic gases xenon and nitrous oxide on urate oxidase: a crystallographic study.,Marassio G, Prange T, David HN, Sopkova-de Oliveira Santos J, Gabison L, Delcroix N, Abraini JH, Colloc'h N FASEB J. 2011 Mar 18. PMID:21421845<ref>PMID:21421845</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[Urate Oxidase|Urate Oxidase]] | *[[Urate Oxidase|Urate Oxidase]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
</StructureSection> | |||
[[Category: Aspergillus flavus]] | [[Category: Aspergillus flavus]] | ||
[[Category: Urate oxidase]] | [[Category: Urate oxidase]] | ||
[[Category: Abraini, J H | [[Category: Abraini, J H]] | ||
[[Category: Marassio, G | [[Category: Marassio, G]] | ||
[[Category: Prange, T | [[Category: Prange, T]] | ||
[[Category: H, N Colloc | [[Category: H, N Colloc]] | ||
[[Category: Oxidase]] | [[Category: Oxidase]] | ||
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
Revision as of 15:24, 9 December 2014
urate oxidase under 1.5 MPa / 15 bars pressure of equimolar mixture xenon : nitrous oxideurate oxidase under 1.5 MPa / 15 bars pressure of equimolar mixture xenon : nitrous oxide
Structural highlights
Publication Abstract from PubMedThe remarkably safe anesthetics xenon (Xe) and, to lesser extent, nitrous oxide (N(2)O) possess neuroprotective properties in preclinical studies. To investigate the mechanisms of pharmacological action of these gases, which are still poorly known, we performed both crystallography under a large range of gas pressure and biochemical studies on urate oxidase, a prototype of globular gas-binding proteins whose activity is modulated by inert gases. We show that Xe and N(2)O bind to, compete for, and expand the volume of a hydrophobic cavity located just behind the active site of urate oxidase and further inhibit urate oxidase enzymatic activity. By demonstrating a significant relationship between the binding and biochemical effects of Xe and N(2)O, given alone or in combination, these data from structure to function highlight the mechanisms by which chemically and metabolically inert gases can alter protein function and produce their pharmacological effects. Interestingly, the effects of a Xe:N(2)O equimolar mixture were found to be equivalent to those of Xe alone, thereby suggesting that gas mixtures containing Xe and N(2)O could be an alternative and efficient neuroprotective strategy to Xe alone, whose widespread clinical use is limited due to the cost of production and availability of this gas.-Marassio, G., Prange, T., David, H. N., Sopkova-de Oliveira Santos, J., Gabison, L., Delcroix, N., Abraini, J. H., Colloc'h, N. Pressure-response analysis of anesthetic gases xenon and nitrous oxide on urate oxidase: a crystallographic study. Pressure-response analysis of anesthetic gases xenon and nitrous oxide on urate oxidase: a crystallographic study.,Marassio G, Prange T, David HN, Sopkova-de Oliveira Santos J, Gabison L, Delcroix N, Abraini JH, Colloc'h N FASEB J. 2011 Mar 18. PMID:21421845[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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