3qap: Difference between revisions
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[[ | ==Crystal structure of Natronomonas pharaonis sensory rhodopsin II in the ground state== | ||
<StructureSection load='3qap' size='340' side='right' caption='[[3qap]], [[Resolution|resolution]] 1.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3qap]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Natronomonas_pharaonis Natronomonas pharaonis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QAP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QAP FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=L2P:2,3-DI-PHYTANYL-GLYCEROL'>L2P</scene>, <scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sop2, sopII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2257 Natronomonas pharaonis])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qap OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qap RCSB], [http://www.ebi.ac.uk/pdbsum/3qap PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The molecular mechanism of transmembrane signal transduction is still a pertinent question in cellular biology. Generally, a receptor can transfer an external signal via its cytoplasmic surface, as found for G-protein-coupled receptors such as rhodopsin, or via the membrane domain, such as that in sensory rhodopsin II (SRII) in complex with its transducer, HtrII. In the absence of HtrII, SRII functions as a proton pump. Here, we report on the crystal structure of the active state of uncomplexed SRII from Natronomonas pharaonis, NpSRII. The problem with a dramatic loss of diffraction quality upon loading of the active state was overcome by growing better crystals and by reducing the occupancy of the state. The conformational changes in the region comprising helices F and G are similar to those observed for the NpSRII-transducer complex but are much more pronounced. The meaning of these differences for the understanding of proton pumping and signal transduction by NpSRII is discussed. | |||
Active State of Sensory Rhodopsin II: Structural Determinants for Signal Transfer and Proton Pumping.,Gushchin I, Reshetnyak A, Borshchevskiy V, Ishchenko A, Round E, Grudinin S, Engelhard M, Buldt G, Gordeliy V J Mol Biol. 2011 Aug 4. PMID:21840321<ref>PMID:21840321</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[Bacteriorhodopsin|Bacteriorhodopsin]] | |||
*[[Rhodopsin|Rhodopsin]] | *[[Rhodopsin|Rhodopsin]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
</StructureSection> | |||
[[Category: Natronomonas pharaonis]] | [[Category: Natronomonas pharaonis]] | ||
[[Category: Borshchevskiy, V | [[Category: Borshchevskiy, V]] | ||
[[Category: Buldt, G | [[Category: Buldt, G]] | ||
[[Category: Engelhard, M | [[Category: Engelhard, M]] | ||
[[Category: Gordeliy, V | [[Category: Gordeliy, V]] | ||
[[Category: Grudinin, S | [[Category: Grudinin, S]] | ||
[[Category: Gushchin, I | [[Category: Gushchin, I]] | ||
[[Category: Ishchenko, A | [[Category: Ishchenko, A]] | ||
[[Category: Reshetnyak, A | [[Category: Reshetnyak, A]] | ||
[[Category: Round, E | [[Category: Round, E]] | ||
[[Category: Membrane]] | [[Category: Membrane]] | ||
[[Category: Nphtrii]] | [[Category: Nphtrii]] | ||
[[Category: Phototaxis]] | [[Category: Phototaxis]] | ||
[[Category: Transport protein]] | [[Category: Transport protein]] | ||