2dd9: Difference between revisions

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-[[Image:2dd9.png|left|200px]]
+==A mutant of GFP-like protein from Chiridius poppei==
+<StructureSection load='2dd9' size='340' side='right' caption='[[2dd9]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2dd9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chiridius_poppei Chiridius poppei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DD9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DD9 FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC+ACID'>CXS</scene><br>
+<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene></td></tr>
+<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2dd7|2dd7]]</td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dd9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2dd9 RCSB], [http://www.ebi.ac.uk/pdbsum/2dd9 PDBsum]</span></td></tr>
+<table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dd/2dd9_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The fluorescence excitation and emission maxima of a GFP-like protein from the marine copepod Chiridius poppei (CpYGFP) show a significant red shift (lambda(ex) = 509 nm, lambda(em) = 517 nm) compared with those of GFP from Aequorea victoria (avGFP) and other GFP-like proteins from marine copepods. We performed crystallographic and biochemical studies to understand why this shift occurs in CpYGFP. The structure of CpYGFP showed that the imidazole side chain of His52 is involved in stacking on the phenol moiety of the chromophore. We investigated the potential role of His52 in causing the red-shifted spectral properties by performing mutational analyses of H52T, H52D and H52F. The emission wavelengths of H52T and H52D were blue-shifted and that of H52F was red-shifted relative to the wild type. Comparison of its structure of another copepod GFP (ppluGFP2) having an emission maximum at 502 nm showed that the imidazole ring of His54 (corresponding to His52 in CpYGFP) is flipped out of the stacking position with the chromophore. These findings suggest that pi-pi stacking interaction between His52 and the phenol moiety of the chromophore is the likely cause of the red-shift in light emission.
-{{STRUCTURE_2dd9|  PDB=2dd9  |  SCENE=  }}
+Structural basis for red-shifted emission of a GFP-like protein from the marine copepod Chiridius poppei.,Suto K, Masuda H, Takenaka Y, Tsuji FI, Mizuno H Genes Cells. 2009 Jun;14(6):727-37. Epub 2009 May 14. PMID:19469881<ref>PMID:19469881</ref>
-===A mutant of GFP-like protein from Chiridius poppei===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_19469881}}
-==About this Structure==
-[[2dd9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chiridius_poppei Chiridius poppei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DD9 OCA].
 ==See Also==
-*[[Alyssa Marsico/Sandbox 1|Alyssa Marsico/Sandbox 1]]
-*[[Devon McCarthy/Sandbox 1|Devon McCarthy/Sandbox 1]]
 *[[Green Fluorescent Protein|Green Fluorescent Protein]]
-*[[Sandbox104|Sandbox104]]
+== References ==
-*[[User:Joanne Lau/Sandbox 5|User:Joanne Lau/Sandbox 5]]
+<references/>
+__TOC__
-==Reference==
+</StructureSection>
-<ref group="xtra">PMID:019469881</ref><references group="xtra"/>
 [[Category: Chiridius poppei]]
 [[Category: Masuda, H.]]