2kif: Difference between revisions

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-[[Image:2kif.png|left|200px]]
+==Solution NMR structure of an O6-methylguanine DNA methyltransferase family protein from Vibrio parahaemolyticus. Northeast Structural Genomics Consortium target VpR247.==
+<StructureSection load='2kif' size='340' side='right' caption='[[2kif]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2kif]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_parahaemolyticus_aq3810 Vibrio parahaemolyticus aq3810]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KIF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KIF FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">A79_1377, VP0951 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=419109 Vibrio parahaemolyticus AQ3810])</td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kif OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kif RCSB], [http://www.ebi.ac.uk/pdbsum/2kif PDBsum]</span></td></tr>
+<table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ki/2kif_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Alkyltransferase-like proteins (ATLs) are a novel class of DNA repair proteins related to O(6)-alkylguanine-DNA alkyltransferases (AGTs) that tightly bind alkylated DNA and shunt the damaged DNA into the nucleotide excision repair pathway. Here, we present the first structure of a bacterial ATL, from Vibrio parahaemolyticus (vpAtl). We demonstrate that vpAtl adopts an AGT-like fold and that the protein is capable of tightly binding to O(6)-methylguanine-containing DNA and disrupting its repair by human AGT, a hallmark of ATLs. Mutation of highly conserved residues Tyr(23) and Arg(37) demonstrate their critical roles in a conserved mechanism of ATL binding to alkylated DNA. NMR relaxation data reveal a role for conformational plasticity in the guanine-lesion recognition cavity. Our results provide further evidence for the conserved role of ATLs in this primordial mechanism of DNA repair.
-{{STRUCTURE_2kif|  PDB=2kif  |  SCENE=  }}
+Structural basis of O6-alkylguanine recognition by a bacterial alkyltransferase-like DNA repair protein.,Aramini JM, Tubbs JL, Kanugula S, Rossi P, Ertekin A, Maglaqui M, Hamilton K, Ciccosanti CT, Jiang M, Xiao R, Soong TT, Rost B, Acton TB, Everett JK, Pegg AE, Tainer JA, Montelione GT J Biol Chem. 2010 Apr 30;285(18):13736-41. Epub 2010 Mar 8. PMID:20212037<ref>PMID:20212037</ref>
-===Solution NMR structure of an O6-methylguanine DNA methyltransferase family protein from Vibrio parahaemolyticus. Northeast Structural Genomics Consortium target VpR247.===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_20212037}}
-==About this Structure==
-[[2kif]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_parahaemolyticus_aq3810 Vibrio parahaemolyticus aq3810]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KIF OCA].
 ==See Also==
 *[[DNA methyltransferase|DNA methyltransferase]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:020212037</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Vibrio parahaemolyticus aq3810]]
 [[Category: Acton, T B.]]