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[[Image:3oyc.png|left|200px]]
==Crystal structure of the Prototype Foamy Virus (PFV) intasome in complex with magnesium and the INSTI PICA==
<StructureSection load='3oyc' size='340' side='right' caption='[[3oyc]], [[Resolution|resolution]] 2.66&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3oyc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_spumaretrovirus Human spumaretrovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OYC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OYC FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=ZZW:9-(4-FLUOROBENZYL)-N-HYDROXY-9H-BETA-CARBOLINE-3-CARBOXAMIDE'>ZZW</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3dlr|3dlr]], [[3l2q|3l2q]], [[3l2r|3l2r]], [[3l2u|3l2u]], [[3l2v|3l2v]], [[3l2w|3l2w]], [[3oy9|3oy9]], [[3oya|3oya]], [[3oyb|3oyb]], [[3oyd|3oyd]], [[3oye|3oye]], [[3oyf|3oyf]], [[3oyg|3oyg]], [[3oyh|3oyh]], [[3oyi|3oyi]], [[3oyj|3oyj]], [[3oyk|3oyk]], [[3oyl|3oyl]], [[3oym|3oym]], [[3oyn|3oyn]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">POL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11963 Human spumaretrovirus])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3oyc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oyc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3oyc RCSB], [http://www.ebi.ac.uk/pdbsum/3oyc PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The development of HIV integrase (IN) strand transfer inhibitors (INSTIs) and our understanding of viral resistance to these molecules have been hampered by a paucity of available structural data. We recently reported cocrystal structures of the prototype foamy virus (PFV) intasome with raltegravir and elvitegravir, establishing the general INSTI binding mode. We now present an expanded set of cocrystal structures containing PFV intasomes complexed with first- and second-generation INSTIs at resolutions of up to 2.5 A. Importantly, the improved resolution allowed us to refine the complete coordination spheres of the catalytic metal cations within the INSTI-bound intasome active site. We show that like the Q148H/G140S and N155H HIV-1 IN variants, the analogous S217H and N224H PFV INs display reduced sensitivity to raltegravir in vitro. Crystal structures of the mutant PFV intasomes in INSTI-free and -bound forms revealed that the amino acid substitutions necessitate considerable conformational rearrangements within the IN active site to accommodate an INSTI, thus explaining their adverse effects on raltegravir antiviral activity. Furthermore, our structures predict physical proximity and an interaction between HIV-1 IN mutant residues His148 and Ser/Ala140, rationalizing the coevolution of Q148H and G140S/A mutations in drug-resistant viral strains.


{{STRUCTURE_3oyc|  PDB=3oyc  |  SCENE=  }}
Molecular mechanisms of retroviral integrase inhibition and the evolution of viral resistance.,Hare S, Vos AM, Clayton RF, Thuring JW, Cummings MD, Cherepanov P Proc Natl Acad Sci U S A. 2010 Oct 28. PMID:21030679<ref>PMID:21030679</ref>


===Crystal structure of the Prototype Foamy Virus (PFV) intasome in complex with magnesium and the INSTI PICA===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
{{ABSTRACT_PUBMED_21030679}}
 
==About this Structure==
[[3oyc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_spumaretrovirus Human spumaretrovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OYC OCA].


==See Also==
==See Also==
*[[Retroviral Integrase|Retroviral Integrase]]
*[[Retroviral Integrase|Retroviral Integrase]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:021030679</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Human spumaretrovirus]]
[[Category: Human spumaretrovirus]]
[[Category: Cherepanov, P.]]
[[Category: Cherepanov, P]]
[[Category: Hare, S.]]
[[Category: Hare, S]]
[[Category: Dna integration]]
[[Category: Dna integration]]
[[Category: Dna-binding]]
[[Category: Dna-binding]]

Revision as of 16:37, 9 December 2014

Crystal structure of the Prototype Foamy Virus (PFV) intasome in complex with magnesium and the INSTI PICACrystal structure of the Prototype Foamy Virus (PFV) intasome in complex with magnesium and the INSTI PICA

Structural highlights

3oyc is a 4 chain structure with sequence from Human spumaretrovirus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:, , , , ,
Gene:POL (Human spumaretrovirus)
Resources:FirstGlance, OCA, RCSB, PDBsum

Publication Abstract from PubMed

The development of HIV integrase (IN) strand transfer inhibitors (INSTIs) and our understanding of viral resistance to these molecules have been hampered by a paucity of available structural data. We recently reported cocrystal structures of the prototype foamy virus (PFV) intasome with raltegravir and elvitegravir, establishing the general INSTI binding mode. We now present an expanded set of cocrystal structures containing PFV intasomes complexed with first- and second-generation INSTIs at resolutions of up to 2.5 A. Importantly, the improved resolution allowed us to refine the complete coordination spheres of the catalytic metal cations within the INSTI-bound intasome active site. We show that like the Q148H/G140S and N155H HIV-1 IN variants, the analogous S217H and N224H PFV INs display reduced sensitivity to raltegravir in vitro. Crystal structures of the mutant PFV intasomes in INSTI-free and -bound forms revealed that the amino acid substitutions necessitate considerable conformational rearrangements within the IN active site to accommodate an INSTI, thus explaining their adverse effects on raltegravir antiviral activity. Furthermore, our structures predict physical proximity and an interaction between HIV-1 IN mutant residues His148 and Ser/Ala140, rationalizing the coevolution of Q148H and G140S/A mutations in drug-resistant viral strains.

Molecular mechanisms of retroviral integrase inhibition and the evolution of viral resistance.,Hare S, Vos AM, Clayton RF, Thuring JW, Cummings MD, Cherepanov P Proc Natl Acad Sci U S A. 2010 Oct 28. PMID:21030679[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Hare S, Vos AM, Clayton RF, Thuring JW, Cummings MD, Cherepanov P. Molecular mechanisms of retroviral integrase inhibition and the evolution of viral resistance. Proc Natl Acad Sci U S A. 2010 Oct 28. PMID:21030679 doi:10.1073/pnas.1010246107

3oyc, resolution 2.66Å

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