2wgz: Difference between revisions

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-[[Image:2wgz.png|left|200px]]
+==CRYSTAL STRUCTURE OF ALPHA-1,3 GALACTOSYLTRANSFERASE (ALPHA3GT) IN A COMPLEX WITH P-NITROPHENYL-BETA-GALACTOSIDE (PNP-BETA-GAL)==
+<StructureSection load='2wgz' size='340' side='right' caption='[[2wgz]], [[Resolution|resolution]] 2.12&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2wgz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WGZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WGZ FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=147:1-O-[P-NITROPHENYL]-BETA-D-GALACTOPYRANOSE'>147</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NPO:P-NITROPHENOL'>NPO</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene><br>
+<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2vs4|2vs4]], [[2vxl|2vxl]], [[1vzt|1vzt]], [[2vxm|2vxm]], [[1gx0|1gx0]], [[1vzx|1vzx]], [[1o7q|1o7q]], [[2vs5|2vs5]], [[1gx4|1gx4]], [[2jck|2jck]], [[2jcj|2jcj]], [[2vfz|2vfz]], [[1g93|1g93]], [[1k4v|1k4v]], [[1g8o|1g8o]], [[1gwv|1gwv]], [[1vzu|1vzu]], [[2vs3|2vs3]], [[1o7o|1o7o]], [[2jcl|2jcl]], [[2jco|2jco]], [[1gww|1gww]], [[1fg5|1fg5]]</td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wgz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wgz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wgz RCSB], [http://www.ebi.ac.uk/pdbsum/2wgz PDBsum]</span></td></tr>
+<table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wg/2wgz_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The specificities of glycosyltransferases make them useful for the synthesis of biologically active oligosaccharides, but also restrict their range of products. In substrate engineering, substrate promiscuity is enhanced by attaching removable interactive groups to weak substrates. Thus, the attachment of betap-nitrophenyl converts galactose from a poor into a good substrate of alpha-1,3-galactosyltransferase. The crystallographic structure of a complex of alpha3GT containing p-nitrophenyl-beta-galactoside shows that the p-nitrophenyl binds similarly to the N-acetylglucosamine of the substrate, N-acetyllactosamine, interacting with the indole of Trp249. p-Nitrophenyl, unlike N-acetylglucosamine, makes no H-bonds but has more non-polar interactions, making it an effective monosaccharide mimetic.
-{{STRUCTURE_2wgz|  PDB=2wgz  |  SCENE=  }}
+Crystal structure of alpha-1,3-galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNPbetaGal).,Jamaluddin H, Tumbale P, Ferns TA, Thiyagarajan N, Brew K, Acharya KR Biochem Biophys Res Commun. 2009 Aug 7;385(4):601-4. Epub 2009 May 30. PMID:19486884<ref>PMID:19486884</ref>
-===CRYSTAL STRUCTURE OF ALPHA-1,3 GALACTOSYLTRANSFERASE (ALPHA3GT) IN A COMPLEX WITH P-NITROPHENYL-BETA-GALACTOSIDE (PNP-BETA-GAL)===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_19486884}}
-==About this Structure==
-[[2wgz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WGZ OCA].
 ==See Also==
-*[[Galactosyltransferase|Galactosyltransferase]]
+*[[Glycosyltransferase|Glycosyltransferase]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:019486884</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Bos taurus]]
 [[Category: Acharya, K R.]]